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Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni

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Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni. / Dillon, R. J. ; Lane, R. P. .

In: Medical and Veterinary Entomology, Vol. 7, No. 3, 07.1993, p. 225-232.

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Dillon, R. J. ; Lane, R. P. . / Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni. In: Medical and Veterinary Entomology. 1993 ; Vol. 7, No. 3. pp. 225-232.

Bibtex

@article{e404638dc52e4d05b8b609b6548d3f39,
title = "Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni",
abstract = "Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2{\%} of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.",
keywords = "Phlebotomus papatasi, Phlebotomus langeroni , sandfly , blood digestion , midgut , protease",
author = "Dillon, {R. J.} and Lane, {R. P.}",
year = "1993",
month = "7",
doi = "10.1111/j.1365-2915.1993.tb00681.x",
language = "English",
volume = "7",
pages = "225--232",
journal = "Medical and Veterinary Entomology",
issn = "0269-283X",
publisher = "Blackwell Publishing Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni

AU - Dillon, R. J.

AU - Lane, R. P.

PY - 1993/7

Y1 - 1993/7

N2 - Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.

AB - Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.

KW - Phlebotomus papatasi

KW - Phlebotomus langeroni

KW - sandfly

KW - blood digestion

KW - midgut

KW - protease

U2 - 10.1111/j.1365-2915.1993.tb00681.x

DO - 10.1111/j.1365-2915.1993.tb00681.x

M3 - Journal article

VL - 7

SP - 225

EP - 232

JO - Medical and Veterinary Entomology

JF - Medical and Veterinary Entomology

SN - 0269-283X

IS - 3

ER -