Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Bloodmeal digestion in the midgut of Phlebotomus papatasi and Phlebotomus langeroni
AU - Dillon, R. J.
AU - Lane, R. P.
PY - 1993/7
Y1 - 1993/7
N2 - Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.
AB - Bloodmeal digestion in midguts of the sandflies Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae) was investigated in optimized assays to detect general protease, trypsin and aminopeptidase activities using synthetic substrates. Optimal activity occurred at pH 8-9 for all enzymes examined in both species. Protease activity peaked at 24-34 h post human bloodmeal in midguts of P.papatasi and 34-48 h in P. langeroni; all endo- and exoprotease activities were completed by 50 h in P.papatasi compared to 72 h in P.langeroni. Hydrolysis of two chymotrypsin substrates was <2% of trypsin activity in both species. Aminopeptidase activity was associated mainly with the midgut wall, whereas trypsin activity was confined to the midgut lumen. A feature of digestion in P.langeroni was the high level of aminopeptidase recorded within 10 h of the bloodmeal.
KW - Phlebotomus papatasi
KW - Phlebotomus langeroni
KW - sandfly
KW - blood digestion
KW - midgut
KW - protease
U2 - 10.1111/j.1365-2915.1993.tb00681.x
DO - 10.1111/j.1365-2915.1993.tb00681.x
M3 - Journal article
VL - 7
SP - 225
EP - 232
JO - Medical and Veterinary Entomology
JF - Medical and Veterinary Entomology
SN - 0269-283X
IS - 3
ER -