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Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Research output: Contribution to journalJournal article

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  • Jennifer Munkley
  • Nikki A Copeland
  • Victoria Moignard
  • John R P Knight
  • Erin Greaves
  • Simon A Ramsbottom
  • Mary E Pownall
  • Jennifer Southgate
  • Justin F-X Ainscough
  • Dawn Coverley
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<mark>Journal publication date</mark>2011
<mark>Journal</mark>Nucleic Acids Research
Issue number7
Volume39
Number of pages7
Pages (from-to)2671-2677
Publication statusPublished
Original languageEnglish

Abstract

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.