Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells
AU - Munkley, Jennifer
AU - Copeland, Nikki A
AU - Moignard, Victoria
AU - Knight, John R P
AU - Greaves, Erin
AU - Ramsbottom, Simon A
AU - Pownall, Mary E
AU - Southgate, Jennifer
AU - Ainscough, Justin F-X
AU - Coverley, Dawn
PY - 2011
Y1 - 2011
N2 - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
AB - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
KW - Active Transport, Cell Nucleus
KW - Animals
KW - Cell Differentiation
KW - Cell Line, Tumor
KW - Cells, Cultured
KW - Cyclin E
KW - Humans
KW - Mice
KW - Neoplasms
KW - Nuclear Matrix
KW - Protein Transport
KW - Xenopus laevis
U2 - 10.1093/nar/gkq1190
DO - 10.1093/nar/gkq1190
M3 - Journal article
C2 - 21109536
VL - 39
SP - 2671
EP - 2677
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 7
ER -