Rights statement: This is the author’s version of a work that was accepted for publication in Reproductive Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Reproductive Toxicology, 62 2016 DOI: 10.1016/j.reprotox.2016.04.016
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Effects of 4-Nonylphenol on spermatogenesis and induction of testicular apoptosis through oxidative stress-related pathways
AU - Duan, Peng
AU - Hu, Chunhui
AU - Butler, Holly
AU - Chen, Wei
AU - Huang, Wenting
AU - Tang, Sha
AU - Zhou, Wei
AU - Yuan, Meng
AU - Shi, Yuqin
AU - Martin, Francis Luke
AU - Yang, Kedi
N1 - This is the author’s version of a work that was accepted for publication in Reproductive Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Reproductive Toxicology, 62 2016 DOI: 10.1016/j.reprotox.2016.04.016
PY - 2016/7/1
Y1 - 2016/7/1
N2 - This study tested the hypothesis that prepubertal exposure to 4-Nonylphenol (NP) affects reproductive function in male rats. Twenty-four rats at five-weeks-old were randomly divided into four groups and treated with NP at varying concentrations (0, 5, 20, and 60 mg/kg/2d) for thirty days by intra-peritoneal injection. 60 mg/kg NP induced spermatogenic degeneration and pronounced deficits in epididymal sperm count, motility and function, whereas potentially stimulatory effects were observed at 5 NP mg/kg. Moreover, 60 mg/kg NP resulted in a significant reduction in fructose, FSH and LH; induced apoptosis related to oxidative stress; inhibited mRNA and protein levels of Bcl-2 and PCNA; as well as the additional up-regulation of p53, Bax, Apaf-1, cytochrome c, cleaved-caspase-3, Fas and FasL expression. Our data suggest potentially hormetic effects of NP on spermatogenic function. High-dose NP impairs testicular development and function by reducing cell proliferation and inducing apoptosis involving oxidative stress-related p53-Bcl-2/Bax and −Fas/FasL pathways.
AB - This study tested the hypothesis that prepubertal exposure to 4-Nonylphenol (NP) affects reproductive function in male rats. Twenty-four rats at five-weeks-old were randomly divided into four groups and treated with NP at varying concentrations (0, 5, 20, and 60 mg/kg/2d) for thirty days by intra-peritoneal injection. 60 mg/kg NP induced spermatogenic degeneration and pronounced deficits in epididymal sperm count, motility and function, whereas potentially stimulatory effects were observed at 5 NP mg/kg. Moreover, 60 mg/kg NP resulted in a significant reduction in fructose, FSH and LH; induced apoptosis related to oxidative stress; inhibited mRNA and protein levels of Bcl-2 and PCNA; as well as the additional up-regulation of p53, Bax, Apaf-1, cytochrome c, cleaved-caspase-3, Fas and FasL expression. Our data suggest potentially hormetic effects of NP on spermatogenic function. High-dose NP impairs testicular development and function by reducing cell proliferation and inducing apoptosis involving oxidative stress-related p53-Bcl-2/Bax and −Fas/FasL pathways.
KW - 4-Nonylphenol
KW - Hormetic effect
KW - Reproductive toxicity
KW - Oxidative stress
KW - Apoptosis
KW - Intrinsic and extrinsic apoptotic pathways
KW - Rats
U2 - 10.1016/j.reprotox.2016.04.016
DO - 10.1016/j.reprotox.2016.04.016
M3 - Journal article
VL - 62
SP - 27
EP - 38
JO - Reproductive Toxicology
JF - Reproductive Toxicology
SN - 0890-6238
ER -