Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence
AU - Possell, Malcolm
AU - Ryan, Annette
AU - Vickers, Claudia
AU - Mullineaux, Philip M.
AU - Hewitt, C. N.
PY - 2010/4
Y1 - 2010/4
N2 - In higher plants, many isoprenoids are synthesised via the chloroplastic 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Attempts to elucidate the function of individual isoprenoids have used the antibiotic/herbicidal compound fosmidomycin (3-[N-formyl-N-hydroxy amino] propyl phosphonic acid) to inhibit this pathway. Examination of the effect of fosmidomycin on the major components of photosynthesis in leaves of white poplar (Populus alba) and tobacco (Nicotiana tabacum) was made. Fosmidomycin reduced net photosynthesis in both species within 1 h of application, but only when photosynthesis was light-saturated. In P. alba, these reductions were confounded by high light and fosmidomycin inducing stomatal patchiness. In tobacco, this was caused by significant reductions in PSII chlorophyll fluorescence and reductions in V (cmax) and J (max). Our data indicate that the diminution of photosynthesis is likely a complex effect resulting from the inhibition of multiple MEP pathway products, resulting in photoinhibition and photo-damage. These effects should be accounted for in experimental design and analysis when using fosmidomycin to avoid misinterpretation of results as measured by gas exchange and chlorophyll fluorescence.
AB - In higher plants, many isoprenoids are synthesised via the chloroplastic 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Attempts to elucidate the function of individual isoprenoids have used the antibiotic/herbicidal compound fosmidomycin (3-[N-formyl-N-hydroxy amino] propyl phosphonic acid) to inhibit this pathway. Examination of the effect of fosmidomycin on the major components of photosynthesis in leaves of white poplar (Populus alba) and tobacco (Nicotiana tabacum) was made. Fosmidomycin reduced net photosynthesis in both species within 1 h of application, but only when photosynthesis was light-saturated. In P. alba, these reductions were confounded by high light and fosmidomycin inducing stomatal patchiness. In tobacco, this was caused by significant reductions in PSII chlorophyll fluorescence and reductions in V (cmax) and J (max). Our data indicate that the diminution of photosynthesis is likely a complex effect resulting from the inhibition of multiple MEP pathway products, resulting in photoinhibition and photo-damage. These effects should be accounted for in experimental design and analysis when using fosmidomycin to avoid misinterpretation of results as measured by gas exchange and chlorophyll fluorescence.
KW - Fosmidomycin
KW - Photosynthesis
KW - Populus alba
KW - Nicotiana tabacum
KW - Isoprenoids
KW - Isoprene
KW - PHRAGMITES-AUSTRALIS LEAVES
KW - 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
KW - ISOPRENOID BIOSYNTHESIS
KW - OXIDATIVE STRESS
KW - TERPENOID BIOSYNTHESIS
KW - MONOTERPENE EMISSIONS
KW - NONMEVALONATE PATHWAY
KW - VOLATILE ISOPRENOIDS
KW - LEAF PHOTOSYNTHESIS
KW - ELECTRON-TRANSPORT
U2 - 10.1007/s11120-009-9504-5
DO - 10.1007/s11120-009-9504-5
M3 - Journal article
VL - 104
SP - 49
EP - 59
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1
ER -