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Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence

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Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence. / Possell, Malcolm; Ryan, Annette; Vickers, Claudia; Mullineaux, Philip M.; Hewitt, C. N.

In: Photosynthesis Research, Vol. 104, No. 1, 04.2010, p. 49-59.

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Possell, Malcolm ; Ryan, Annette ; Vickers, Claudia ; Mullineaux, Philip M. ; Hewitt, C. N. / Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence. In: Photosynthesis Research. 2010 ; Vol. 104, No. 1. pp. 49-59.

Bibtex

@article{5deb96c016e94dcfa5d1bfa2482c3944,
title = "Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence",
abstract = "In higher plants, many isoprenoids are synthesised via the chloroplastic 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Attempts to elucidate the function of individual isoprenoids have used the antibiotic/herbicidal compound fosmidomycin (3-[N-formyl-N-hydroxy amino] propyl phosphonic acid) to inhibit this pathway. Examination of the effect of fosmidomycin on the major components of photosynthesis in leaves of white poplar (Populus alba) and tobacco (Nicotiana tabacum) was made. Fosmidomycin reduced net photosynthesis in both species within 1 h of application, but only when photosynthesis was light-saturated. In P. alba, these reductions were confounded by high light and fosmidomycin inducing stomatal patchiness. In tobacco, this was caused by significant reductions in PSII chlorophyll fluorescence and reductions in V (cmax) and J (max). Our data indicate that the diminution of photosynthesis is likely a complex effect resulting from the inhibition of multiple MEP pathway products, resulting in photoinhibition and photo-damage. These effects should be accounted for in experimental design and analysis when using fosmidomycin to avoid misinterpretation of results as measured by gas exchange and chlorophyll fluorescence.",
keywords = "Fosmidomycin, Photosynthesis, Populus alba, Nicotiana tabacum, Isoprenoids, Isoprene, PHRAGMITES-AUSTRALIS LEAVES, 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE, ISOPRENOID BIOSYNTHESIS, OXIDATIVE STRESS, TERPENOID BIOSYNTHESIS, MONOTERPENE EMISSIONS, NONMEVALONATE PATHWAY, VOLATILE ISOPRENOIDS, LEAF PHOTOSYNTHESIS, ELECTRON-TRANSPORT",
author = "Malcolm Possell and Annette Ryan and Claudia Vickers and Mullineaux, {Philip M.} and Hewitt, {C. N.}",
year = "2010",
month = apr,
doi = "10.1007/s11120-009-9504-5",
language = "English",
volume = "104",
pages = "49--59",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "1",

}

RIS

TY - JOUR

T1 - Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence

AU - Possell, Malcolm

AU - Ryan, Annette

AU - Vickers, Claudia

AU - Mullineaux, Philip M.

AU - Hewitt, C. N.

PY - 2010/4

Y1 - 2010/4

N2 - In higher plants, many isoprenoids are synthesised via the chloroplastic 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Attempts to elucidate the function of individual isoprenoids have used the antibiotic/herbicidal compound fosmidomycin (3-[N-formyl-N-hydroxy amino] propyl phosphonic acid) to inhibit this pathway. Examination of the effect of fosmidomycin on the major components of photosynthesis in leaves of white poplar (Populus alba) and tobacco (Nicotiana tabacum) was made. Fosmidomycin reduced net photosynthesis in both species within 1 h of application, but only when photosynthesis was light-saturated. In P. alba, these reductions were confounded by high light and fosmidomycin inducing stomatal patchiness. In tobacco, this was caused by significant reductions in PSII chlorophyll fluorescence and reductions in V (cmax) and J (max). Our data indicate that the diminution of photosynthesis is likely a complex effect resulting from the inhibition of multiple MEP pathway products, resulting in photoinhibition and photo-damage. These effects should be accounted for in experimental design and analysis when using fosmidomycin to avoid misinterpretation of results as measured by gas exchange and chlorophyll fluorescence.

AB - In higher plants, many isoprenoids are synthesised via the chloroplastic 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Attempts to elucidate the function of individual isoprenoids have used the antibiotic/herbicidal compound fosmidomycin (3-[N-formyl-N-hydroxy amino] propyl phosphonic acid) to inhibit this pathway. Examination of the effect of fosmidomycin on the major components of photosynthesis in leaves of white poplar (Populus alba) and tobacco (Nicotiana tabacum) was made. Fosmidomycin reduced net photosynthesis in both species within 1 h of application, but only when photosynthesis was light-saturated. In P. alba, these reductions were confounded by high light and fosmidomycin inducing stomatal patchiness. In tobacco, this was caused by significant reductions in PSII chlorophyll fluorescence and reductions in V (cmax) and J (max). Our data indicate that the diminution of photosynthesis is likely a complex effect resulting from the inhibition of multiple MEP pathway products, resulting in photoinhibition and photo-damage. These effects should be accounted for in experimental design and analysis when using fosmidomycin to avoid misinterpretation of results as measured by gas exchange and chlorophyll fluorescence.

KW - Fosmidomycin

KW - Photosynthesis

KW - Populus alba

KW - Nicotiana tabacum

KW - Isoprenoids

KW - Isoprene

KW - PHRAGMITES-AUSTRALIS LEAVES

KW - 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE

KW - ISOPRENOID BIOSYNTHESIS

KW - OXIDATIVE STRESS

KW - TERPENOID BIOSYNTHESIS

KW - MONOTERPENE EMISSIONS

KW - NONMEVALONATE PATHWAY

KW - VOLATILE ISOPRENOIDS

KW - LEAF PHOTOSYNTHESIS

KW - ELECTRON-TRANSPORT

U2 - 10.1007/s11120-009-9504-5

DO - 10.1007/s11120-009-9504-5

M3 - Journal article

VL - 104

SP - 49

EP - 59

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 1

ER -