Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus
AU - Robinson, Nicholas P.
AU - Dionne, Isabelle
AU - Lundgren, Magnus
AU - Marsh, Victoria L.
AU - Bernander, Rolf
AU - Bell, Stephen D.
PY - 2004/1/9
Y1 - 2004/1/9
N2 - Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.
AB - Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.
KW - Archaeal Proteins
KW - Binding Sites
KW - Cell Cycle Proteins
KW - Chromosome Mapping
KW - Chromosomes, Archaeal
KW - DNA Replication
KW - DNA, Complementary
KW - DNA-Binding Proteins
KW - Evolution, Molecular
KW - Molecular Sequence Data
KW - Origin Recognition Complex
KW - Protein Binding
KW - Replication Origin
KW - Saccharomyces cerevisiae Proteins
KW - Sequence Homology, Amino Acid
KW - Sequence Homology, Nucleic Acid
KW - Sulfolobus
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/S0092-8674(03)01034-1
DO - 10.1016/S0092-8674(03)01034-1
M3 - Journal article
C2 - 14718164
VL - 116
SP - 25
EP - 38
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -