Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus

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Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. / Robinson, Nicholas P.; Dionne, Isabelle; Lundgren, Magnus et al.
In: Cell, Vol. 116, No. 1, 09.01.2004, p. 25-38.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Robinson, NP, Dionne, I, Lundgren, M, Marsh, VL, Bernander, R & Bell, SD 2004, 'Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus', Cell, vol. 116, no. 1, pp. 25-38. https://doi.org/10.1016/S0092-8674(03)01034-1

APA

Robinson, N. P., Dionne, I., Lundgren, M., Marsh, V. L., Bernander, R., & Bell, S. D. (2004). Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. Cell, 116(1), 25-38. https://doi.org/10.1016/S0092-8674(03)01034-1

Vancouver

Robinson NP, Dionne I, Lundgren M, Marsh VL, Bernander R, Bell SD. Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. Cell. 2004 Jan 9;116(1):25-38. doi: 10.1016/S0092-8674(03)01034-1

Author

Robinson, Nicholas P. ; Dionne, Isabelle ; Lundgren, Magnus et al. / Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. In: Cell. 2004 ; Vol. 116, No. 1. pp. 25-38.

Bibtex

@article{3f223adf482b4d6faf650ac0e8daf8b5,

title = "Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus",

abstract = "Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.",

keywords = "Archaeal Proteins, Binding Sites, Cell Cycle Proteins, Chromosome Mapping, Chromosomes, Archaeal, DNA Replication, DNA, Complementary, DNA-Binding Proteins, Evolution, Molecular, Molecular Sequence Data, Origin Recognition Complex, Protein Binding, Replication Origin, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Sulfolobus, Journal Article, Research Support, Non-U.S. Gov't",

author = "Robinson, {Nicholas P.} and Isabelle Dionne and Magnus Lundgren and Marsh, {Victoria L.} and Rolf Bernander and Bell, {Stephen D.}",

year = "2004",

month = jan,

day = "9",

doi = "10.1016/S0092-8674(03)01034-1",

language = "English",

volume = "116",

pages = "25--38",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

RIS

TY - JOUR

T1 - Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus

AU - Robinson, Nicholas P.

AU - Dionne, Isabelle

AU - Lundgren, Magnus

AU - Marsh, Victoria L.

AU - Bernander, Rolf

AU - Bell, Stephen D.

PY - 2004/1/9

Y1 - 2004/1/9

N2 - Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.

AB - Eukaryotic chromosomes possess multiple origins of replication, whereas bacterial chromosomes are replicated from a single origin. The archaeon Pyrococcus abyssi also appears to have a single origin, suggesting a common rule for prokaryotes. However, in the current work, we describe the identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus. Further, we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc1 and Cdc6. We demonstrate that the two origins are recognized by distinct subsets of these Orc1/Cdc6 homologs. These data, in conjunction with an analysis of the levels of the three Orc1/Cdc6 proteins in different growth phases and cell cycle stages, lead us to propose a model for the roles for these proteins in modulating origin activity.

KW - Archaeal Proteins

KW - Binding Sites

KW - Cell Cycle Proteins

KW - Chromosome Mapping

KW - Chromosomes, Archaeal

KW - DNA Replication

KW - DNA, Complementary

KW - DNA-Binding Proteins

KW - Evolution, Molecular

KW - Molecular Sequence Data

KW - Origin Recognition Complex

KW - Protein Binding

KW - Replication Origin

KW - Saccharomyces cerevisiae Proteins

KW - Sequence Homology, Amino Acid

KW - Sequence Homology, Nucleic Acid

KW - Sulfolobus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/S0092-8674(03)01034-1

DO - 10.1016/S0092-8674(03)01034-1

M3 - Journal article

C2 - 14718164

VL - 116

SP - 25

EP - 38

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -

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