Accepted author manuscript, 2.7 MB, PDF document
Available under license: CC BY: Creative Commons Attribution 4.0 International License
Accepted author manuscript, 4.01 MB, PDF document
Available under license: CC BY: Creative Commons Attribution 4.0 International License
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL
AU - Halliwell, Diane
AU - Morais, Camilo L. M.
AU - Gomes De Lima, Kassio Michell
AU - Trevisan, Julio
AU - Siggel-King, Michele R. F.
AU - Craig, Tim
AU - Ingham, James
AU - Martin, David S.
AU - Heys, Kelly
AU - Kyrgiou, Maria
AU - Mitra, Anita
AU - Paraskevaidis, Evangelos
AU - Theophilou, Georgios
AU - Martin-Hirsch, Pierre Leonard
AU - Cricenti, Antonio
AU - Luce, Marco
AU - Weightman, Peter
AU - Martin, Francis Luke
PY - 2016/7/12
Y1 - 2016/7/12
N2 - Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.
AB - Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.
U2 - 10.1038/srep29494
DO - 10.1038/srep29494
M3 - Journal article
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 29494
ER -