Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Investigation of ionic liquids as a pretreatment solvent for extraction of collagen biopolymer from waste fish scales using COSMO-RS and experiment
AU - Muhammad, N.
AU - Gonfa, G.
AU - Rahim, A.
AU - Ahmad, P.
AU - Iqbal, F.
AU - Sharif, F.
AU - Khan, A.S.
AU - Khan, F.U.
AU - Khan, Z.U.H.
AU - Rehman, F.
AU - Rehman, I.U.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Collagen being an abundant natural biopolymer and also has many applications for bio-medical and non-biomedical uses, were extracted from waste fish scales using green route of ionic liquid pretreatment. To get insight on dissolution and extraction of collagen from fish scale using ionic liquids, COSMO-RS computational approach was used to identify the best ionic liquid. The 1-ethyl-3-methylimidazolium acetate ([C2C1im][Ac]) ionic liquid based on low activity coefficient (inverse of solubility) and best fit sigma profile was selected for pretreatment. The ground fish scales were pretreated with [C2C1im][Ac] ionic liquid and further subjected to separation for obtaining the collagen. The collagen was obtained in the form of thin film with yield of 3.1 ± 0.5% at 100 °C for 12 h treatment time. The obtained collagen was characterized using Fourier Transform Infrared Spectroscopy (FTIR), UV–Vis spectrometer, Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX). The FTIR, UV–Vis and XRD analysis shows the characteristic peaks assigned to the collagen. The SEM analysis confirms the dissolution of collagen fibers in ionic liquid. The TGA analysis shows lower thermal stability of extracted collagen as compared to fish scales.
AB - Collagen being an abundant natural biopolymer and also has many applications for bio-medical and non-biomedical uses, were extracted from waste fish scales using green route of ionic liquid pretreatment. To get insight on dissolution and extraction of collagen from fish scale using ionic liquids, COSMO-RS computational approach was used to identify the best ionic liquid. The 1-ethyl-3-methylimidazolium acetate ([C2C1im][Ac]) ionic liquid based on low activity coefficient (inverse of solubility) and best fit sigma profile was selected for pretreatment. The ground fish scales were pretreated with [C2C1im][Ac] ionic liquid and further subjected to separation for obtaining the collagen. The collagen was obtained in the form of thin film with yield of 3.1 ± 0.5% at 100 °C for 12 h treatment time. The obtained collagen was characterized using Fourier Transform Infrared Spectroscopy (FTIR), UV–Vis spectrometer, Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX). The FTIR, UV–Vis and XRD analysis shows the characteristic peaks assigned to the collagen. The SEM analysis confirms the dissolution of collagen fibers in ionic liquid. The TGA analysis shows lower thermal stability of extracted collagen as compared to fish scales.
KW - Characterization
KW - Collagen
KW - COSMO-RS analysis
KW - Extraction
KW - Ionic liquid
KW - Biomolecules
KW - Dissolution
KW - Fish
KW - Fourier transform infrared spectroscopy
KW - Gravimetric analysis
KW - Ionic liquids
KW - Liquids
KW - Spectrometers
KW - Thermodynamic stability
KW - Thermogravimetric analysis
KW - X ray diffraction
KW - 1-ethyl-3-methylimidazolium acetates
KW - Characteristic peaks
KW - Computational approach
KW - COSMO-RS
KW - Energy dispersive x-ray
KW - Field emission scanning
KW - Ionic liquid pretreatment
KW - Thermal gravimetric analyses (TGA)
U2 - 10.1016/j.molliq.2017.02.083
DO - 10.1016/j.molliq.2017.02.083
M3 - Journal article
VL - 232
SP - 258
EP - 264
JO - Journal of Molecular Liquids
JF - Journal of Molecular Liquids
SN - 0167-7322
ER -