TY - JOUR

T1 - Investigation of ionic liquids as a pretreatment solvent for extraction of collagen biopolymer from waste fish scales using COSMO-RS and experiment

AU - Muhammad, N.

AU - Gonfa, G.

AU - Rahim, A.

AU - Ahmad, P.

AU - Iqbal, F.

AU - Sharif, F.

AU - Khan, A.S.

AU - Khan, F.U.

AU - Khan, Z.U.H.

AU - Rehman, F.

AU - Rehman, I.U.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Collagen being an abundant natural biopolymer and also has many applications for bio-medical and non-biomedical uses, were extracted from waste fish scales using green route of ionic liquid pretreatment. To get insight on dissolution and extraction of collagen from fish scale using ionic liquids, COSMO-RS computational approach was used to identify the best ionic liquid. The 1-ethyl-3-methylimidazolium acetate ([C2C1im][Ac]) ionic liquid based on low activity coefficient (inverse of solubility) and best fit sigma profile was selected for pretreatment. The ground fish scales were pretreated with [C2C1im][Ac] ionic liquid and further subjected to separation for obtaining the collagen. The collagen was obtained in the form of thin film with yield of 3.1 ± 0.5% at 100 °C for 12 h treatment time. The obtained collagen was characterized using Fourier Transform Infrared Spectroscopy (FTIR), UV–Vis spectrometer, Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX). The FTIR, UV–Vis and XRD analysis shows the characteristic peaks assigned to the collagen. The SEM analysis confirms the dissolution of collagen fibers in ionic liquid. The TGA analysis shows lower thermal stability of extracted collagen as compared to fish scales.

AB - Collagen being an abundant natural biopolymer and also has many applications for bio-medical and non-biomedical uses, were extracted from waste fish scales using green route of ionic liquid pretreatment. To get insight on dissolution and extraction of collagen from fish scale using ionic liquids, COSMO-RS computational approach was used to identify the best ionic liquid. The 1-ethyl-3-methylimidazolium acetate ([C2C1im][Ac]) ionic liquid based on low activity coefficient (inverse of solubility) and best fit sigma profile was selected for pretreatment. The ground fish scales were pretreated with [C2C1im][Ac] ionic liquid and further subjected to separation for obtaining the collagen. The collagen was obtained in the form of thin film with yield of 3.1 ± 0.5% at 100 °C for 12 h treatment time. The obtained collagen was characterized using Fourier Transform Infrared Spectroscopy (FTIR), UV–Vis spectrometer, Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX). The FTIR, UV–Vis and XRD analysis shows the characteristic peaks assigned to the collagen. The SEM analysis confirms the dissolution of collagen fibers in ionic liquid. The TGA analysis shows lower thermal stability of extracted collagen as compared to fish scales.

KW - Characterization

KW - Collagen

KW - COSMO-RS analysis

KW - Extraction

KW - Ionic liquid

KW - Biomolecules

KW - Dissolution

KW - Fish

KW - Fourier transform infrared spectroscopy

KW - Gravimetric analysis

KW - Ionic liquids

KW - Liquids

KW - Spectrometers

KW - Thermodynamic stability

KW - Thermogravimetric analysis

KW - X ray diffraction

KW - 1-ethyl-3-methylimidazolium acetates

KW - Characteristic peaks

KW - Computational approach

KW - COSMO-RS

KW - Energy dispersive x-ray

KW - Field emission scanning

KW - Ionic liquid pretreatment

KW - Thermal gravimetric analyses (TGA)

U2 - 10.1016/j.molliq.2017.02.083

DO - 10.1016/j.molliq.2017.02.083

M3 - Journal article

VL - 232

SP - 258

EP - 264

JO - Journal of Molecular Liquids

JF - Journal of Molecular Liquids

SN - 0167-7322

ER -