Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Isoprene synthesis in plants: lessons from a transgenic tobacco model
AU - Vickers, Claudia E.
AU - Possell, Malcolm
AU - Laothawornkitkul, Jullada
AU - Ryan, Annette
AU - Hewitt, C. N.
AU - Mullineaux, Philip M.
PY - 2011/6
Y1 - 2011/6
N2 - Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.
AB - Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.
KW - chloroplast
KW - circadian rhythms
KW - isoprene synthase
KW - GREY POPLAR LEAVES
KW - VOLATILE ISOPRENOIDS
KW - GENE-EXPRESSION
KW - DIMETHYLALLYL DIPHOSPHATE
KW - BIOGENIC ISOPRENE
KW - CIRCADIAN CONTROL
KW - SYNTHASE ACTIVITY
KW - EMISSION FACTOR
KW - QUERCUS-ROBUR
KW - P-31 NMR
U2 - 10.1111/j.1365-3040.2011.02303.x
DO - 10.1111/j.1365-3040.2011.02303.x
M3 - Journal article
VL - 34
SP - 1043
EP - 1053
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 6
ER -