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Isoprene synthesis in plants: lessons from a transgenic tobacco model

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Isoprene synthesis in plants: lessons from a transgenic tobacco model. / Vickers, Claudia E.; Possell, Malcolm; Laothawornkitkul, Jullada et al.
In: Plant, Cell and Environment, Vol. 34, No. 6, 06.2011, p. 1043-1053.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Vickers, CE, Possell, M, Laothawornkitkul, J, Ryan, A, Hewitt, CN & Mullineaux, PM 2011, 'Isoprene synthesis in plants: lessons from a transgenic tobacco model', Plant, Cell and Environment, vol. 34, no. 6, pp. 1043-1053. https://doi.org/10.1111/j.1365-3040.2011.02303.x

APA

Vickers, C. E., Possell, M., Laothawornkitkul, J., Ryan, A., Hewitt, C. N., & Mullineaux, P. M. (2011). Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant, Cell and Environment, 34(6), 1043-1053. https://doi.org/10.1111/j.1365-3040.2011.02303.x

Vancouver

Vickers CE, Possell M, Laothawornkitkul J, Ryan A, Hewitt CN, Mullineaux PM. Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant, Cell and Environment. 2011 Jun;34(6):1043-1053. doi: 10.1111/j.1365-3040.2011.02303.x

Author

Vickers, Claudia E. ; Possell, Malcolm ; Laothawornkitkul, Jullada et al. / Isoprene synthesis in plants: lessons from a transgenic tobacco model. In: Plant, Cell and Environment. 2011 ; Vol. 34, No. 6. pp. 1043-1053.

Bibtex

@article{8a675b1854844beaabe2d4dd00394314,
title = "Isoprene synthesis in plants: lessons from a transgenic tobacco model",
abstract = "Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.",
keywords = "chloroplast, circadian rhythms, isoprene synthase, GREY POPLAR LEAVES, VOLATILE ISOPRENOIDS, GENE-EXPRESSION, DIMETHYLALLYL DIPHOSPHATE, BIOGENIC ISOPRENE, CIRCADIAN CONTROL, SYNTHASE ACTIVITY, EMISSION FACTOR, QUERCUS-ROBUR, P-31 NMR",
author = "Vickers, {Claudia E.} and Malcolm Possell and Jullada Laothawornkitkul and Annette Ryan and Hewitt, {C. N.} and Mullineaux, {Philip M.}",
year = "2011",
month = jun,
doi = "10.1111/j.1365-3040.2011.02303.x",
language = "English",
volume = "34",
pages = "1043--1053",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Isoprene synthesis in plants: lessons from a transgenic tobacco model

AU - Vickers, Claudia E.

AU - Possell, Malcolm

AU - Laothawornkitkul, Jullada

AU - Ryan, Annette

AU - Hewitt, C. N.

AU - Mullineaux, Philip M.

PY - 2011/6

Y1 - 2011/6

N2 - Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.

AB - Isoprene is a highly reactive gas, and is emitted in such large quantities from the biosphere that it substantially affects the oxidizing potential of the atmosphere. Relatively little is known about the control of isoprene emission at the molecular level. Using transgenic tobacco lines harbouring a poplar isoprene synthase gene, we examined control of isoprene emission. Isoprene synthase required chloroplastic localization for catalytic activity, and isoprene was produced via the methyl erythritol (MEP) pathway from recently assimilated carbon. Emission patterns in transgenic tobacco plants were remarkably similar to naturally emitting plants under a wide variety of conditions. Emissions correlated with photosynthetic rates in developing and mature leaves, and with the amount of isoprene synthase protein in mature leaves. Isoprene synthase protein levels did not change under short-term increase in heat/light, despite an increase in emissions under these conditions. A robust circadian pattern could be observed in emissions from long-day plants. The data support the idea that substrate supply and changes in enzyme kinetics (rather than changes in isoprene synthase levels or post-translational regulation of activity) are the primary controls on isoprene emission in mature transgenic tobacco leaves.

KW - chloroplast

KW - circadian rhythms

KW - isoprene synthase

KW - GREY POPLAR LEAVES

KW - VOLATILE ISOPRENOIDS

KW - GENE-EXPRESSION

KW - DIMETHYLALLYL DIPHOSPHATE

KW - BIOGENIC ISOPRENE

KW - CIRCADIAN CONTROL

KW - SYNTHASE ACTIVITY

KW - EMISSION FACTOR

KW - QUERCUS-ROBUR

KW - P-31 NMR

U2 - 10.1111/j.1365-3040.2011.02303.x

DO - 10.1111/j.1365-3040.2011.02303.x

M3 - Journal article

VL - 34

SP - 1043

EP - 1053

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 6

ER -