We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK


93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > 13C-NMR spectroscopy of keratan sulphates
View graph of relations

« Back

<sup>13</sup>C-NMR spectroscopy of keratan sulphates

Research output: Contribution to journalJournal article


Journal publication date1995
JournalEuropean Journal of Biochemistry
Journal number3
Number of pages5
Original languageEnglish


Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete <sup>13</sup>C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using <sup>13</sup>C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.