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<sup>13</sup>C-NMR spectroscopy of keratan sulphates

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<sup>13</sup>C-NMR spectroscopy of keratan sulphates. / Huckerby, Thomas N.; Brown, Gavin M.; Nieduszynski, Ian A.

In: European Journal of Biochemistry, Vol. 231, No. 3, 1995, p. 779-783.

Research output: Contribution to Journal/MagazineJournal article

Harvard

Huckerby, TN, Brown, GM & Nieduszynski, IA 1995, '<sup>13</sup>C-NMR spectroscopy of keratan sulphates', European Journal of Biochemistry, vol. 231, no. 3, pp. 779-783.

APA

Huckerby, T. N., Brown, G. M., & Nieduszynski, I. A. (1995). <sup>13</sup>C-NMR spectroscopy of keratan sulphates. European Journal of Biochemistry, 231(3), 779-783.

Vancouver

Huckerby TN, Brown GM, Nieduszynski IA. <sup>13</sup>C-NMR spectroscopy of keratan sulphates. European Journal of Biochemistry. 1995;231(3):779-783.

Author

Huckerby, Thomas N. ; Brown, Gavin M. ; Nieduszynski, Ian A. / <sup>13</sup>C-NMR spectroscopy of keratan sulphates. In: European Journal of Biochemistry. 1995 ; Vol. 231, No. 3. pp. 779-783.

Bibtex

@article{455b8a224bfb4c72917a74f8406f968e,
title = "13C-NMR spectroscopy of keratan sulphates",
abstract = "Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete 13C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using 13C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.",
author = "Huckerby, {Thomas N.} and Brown, {Gavin M.} and Nieduszynski, {Ian A.}",
year = "1995",
language = "English",
volume = "231",
pages = "779--783",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - 13C-NMR spectroscopy of keratan sulphates

AU - Huckerby, Thomas N.

AU - Brown, Gavin M.

AU - Nieduszynski, Ian A.

PY - 1995

Y1 - 1995

N2 - Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete 13C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using 13C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.

AB - Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete 13C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using 13C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.

M3 - Journal article

VL - 231

SP - 779

EP - 783

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 3

ER -