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Measurement of cytosolic-free Ca2+ in plant tissue

Research output: Contribution in Book/Report/ProceedingsChapter

Published

Publication date2013
Host publicationCalcium signalling protocols
EditorsDavid Lambert, Richard Rainbow
Place of publicationUSA
PublisherHumana Press
Pages327-341
Number of pages15
Edition3rd
ISBN (Print)978-1-58829-442-5
Original languageEnglish

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, USA
Volume937
ISSN (Print)1064-3745

Abstract

A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2