Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Membrane raft actin deficiency and altered Ca2+-induced vesiculation in stomatin-deficient overhydrated hereditary stomatocytosis
AU - Wilkinson, D Katie
AU - Turner, E Jane
AU - Parkin, Edward T
AU - Garner, Ashley E
AU - Harrison, Penny J
AU - Crawford, Mark
AU - Stewart, Gordon W
AU - Hooper, Nigel M
PY - 2008/1
Y1 - 2008/1
N2 - In overhydrated hereditary stomatocytosis (OHSt), the membrane raft-associated stomatin is deficient from the erythrocyte membrane. We have investigated two aspects of raft structure and function in OHSt erythrocytes. First, we have studied the distribution of other membrane and cytoskeletal proteins in rafts by analysis of detergent-resistant membranes (DRMs). In normal erythrocytes, 29% of the actin was DRM-associated, whereas in two unrelated OHSt patients the DRM-associated actin was reduced to
AB - In overhydrated hereditary stomatocytosis (OHSt), the membrane raft-associated stomatin is deficient from the erythrocyte membrane. We have investigated two aspects of raft structure and function in OHSt erythrocytes. First, we have studied the distribution of other membrane and cytoskeletal proteins in rafts by analysis of detergent-resistant membranes (DRMs). In normal erythrocytes, 29% of the actin was DRM-associated, whereas in two unrelated OHSt patients the DRM-associated actin was reduced to
KW - Actins
KW - Anemia, Hemolytic
KW - Animals
KW - Blood Proteins
KW - Calcium
KW - Cell Line
KW - Cytoskeletal Proteins
KW - Detergents
KW - Dogs
KW - Electrophoresis, Polyacrylamide Gel
KW - Erythrocytes
KW - Exocytosis
KW - Humans
KW - Membrane Microdomains
KW - Membrane Proteins
KW - Microscopy, Atomic Force
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Tropomodulin
U2 - 10.1016/j.bbamem.2007.09.016
DO - 10.1016/j.bbamem.2007.09.016
M3 - Journal article
C2 - 17961506
VL - 1778
SP - 125
EP - 132
JO - Biochimica et Biophysica Acta (BBA) - Biomembranes
JF - Biochimica et Biophysica Acta (BBA) - Biomembranes
SN - 0005-2736
IS - 1
ER -