Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Novel tertiary amine oxide surfaces that resist nonspecific protein adsorption
AU - Dilly, Suzanne J.
AU - Beecham, Matthew P.
AU - Brown, Steven P.
AU - Griffin, John M.
AU - Clark, Andrew J.
AU - Griffin, Craig D.
AU - Marshall, Jacqueline
AU - Napier, Richard M.
AU - Taylor, Paul C.
AU - Marsh, Andrew
PY - 2006/9/12
Y1 - 2006/9/12
N2 - Novel surfaces derivatized with tertiary amine oxides have been prepared and tested for their ability to resist nonspecific protein adsorption. The oxidation of tertiary amines supported on triazine units was carried out using mCPBA to give a format allowing conjugation of biologically active ligands alongside them. Adsorption to these surfaces was tested and compared to adsorption to a set of commercial and custom oligo-/poly(ethylene glycol) (OEG/PEG) supports by challenging them with a protein display library presented on bacteriophage I. The new class of amine oxide surfaces is found to compare favorably with the performance of the OEG/PEG supports in the prevention of nonspecific binding.
AB - Novel surfaces derivatized with tertiary amine oxides have been prepared and tested for their ability to resist nonspecific protein adsorption. The oxidation of tertiary amines supported on triazine units was carried out using mCPBA to give a format allowing conjugation of biologically active ligands alongside them. Adsorption to these surfaces was tested and compared to adsorption to a set of commercial and custom oligo-/poly(ethylene glycol) (OEG/PEG) supports by challenging them with a protein display library presented on bacteriophage I. The new class of amine oxide surfaces is found to compare favorably with the performance of the OEG/PEG supports in the prevention of nonspecific binding.
KW - SELF-ASSEMBLED MONOLAYERS
KW - POLY(ETHYLENE GLYCOL)
KW - CROSS-POLARIZATION
KW - GOLD
KW - BIOMATERIALS
KW - COSOLVENTS
KW - STABILITY
KW - DENSITY
KW - NMR
U2 - 10.1021/la060743j
DO - 10.1021/la060743j
M3 - Journal article
VL - 22
SP - 8144
EP - 8150
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 19
ER -