Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models
AU - Yorucu, C.
AU - Lau, K.
AU - Mittar, S.
AU - Green, N.H.
AU - Raza, A.
AU - Rehman, I.U.
AU - MacNeil, S.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue. © 2016 Ceyla Yorucu, Katherine Lau, Shweta Mittar, Nicola H. Green, Ahtasham Raza, Ihtesham Ur Rehman, and Sheila MacNeil.
AB - Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue. © 2016 Ceyla Yorucu, Katherine Lau, Shweta Mittar, Nicola H. Green, Ahtasham Raza, Ihtesham Ur Rehman, and Sheila MacNeil.
KW - melanoma
KW - metastases
KW - Raman spectroscopy
KW - tissue engineering
KW - Amino acids
KW - Dermatology
KW - Oncology
KW - Principal component analysis
KW - Tissue engineering
KW - Tumors
KW - Extracellular matrices
KW - Melanoma cells
KW - Melanoma tumors
KW - Normal skin
KW - Normal tissue
KW - Protein conformation
KW - Tissue
U2 - 10.1080/05704928.2015.1126840
DO - 10.1080/05704928.2015.1126840
M3 - Journal article
VL - 51
SP - 263
EP - 277
JO - APPLIED SPECTROSCOPY REVIEWS
JF - APPLIED SPECTROSCOPY REVIEWS
SN - 0570-4928
IS - 4
ER -