This paper presents findings from a comprehensive field campaign of stream flow measurement in two Chalk catchments in Berkshire (UK). The measurements were taken in order to characterise the spatial and temporal dynamics of groundwater–surface water interaction within the area, and facilitate a better understanding of underlying processes with the aid of supporting groundwater and water-chemistry data. Long-term streamflow data are used to approximate the baseflow component at three sites within each catchment. Spot-measured streamflow data are then used to describe the seasonal variability in streamflow, at a number of locations within each catchment, in order to determine the spatial and temporal dynamics of areas of groundwater–surface interaction. This study was completed as part of the lowland catchment research programme (LOCAR) and as such aimed to improve monitoring techniques for use within water resource management. In particular, the methods employed provide a useful template for characterisation of similar type catchments and which may be used to ensure effective implementation of Water Framework Directive related environmental assessments.