12,000

We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK

93%

93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > Synthetic biology approach for mining new enzym...
View graph of relations

« Back

Synthetic biology approach for mining new enzymes from unculturable microorganisms

Research output: Contribution to journalMeeting abstract

Published

Journal publication date04/2010
JournalLuminescence
Journal number2
Volume25
Number of pages1
Pages104
Original languageEnglish

Abstract

Synthetic biology is aimed to design and rewire existing and new biological parts, devices, and systems for useful purposes. The vast majority of microbes (>99%) in natural environment are not yet culturable using traditional approaches. Those unculturable microorganisms in natural environment are an untapped gene
resource which is believed to encode novel industrial catalysts and enzymes, as well as new medical compounds. We developed a novel synthetic biology approach that employs a gene trapping circuit to screen functional genes encoded with special enzymes. Using this approach, we have discovered fi ve new naphthalene and toluene dioxygenases which can be used as industrial biocatalysts such as synthesis of HIV inhibitor. This synthetic biology approach paved the way for mining the gene pool of unculturable microorganisms and searching novel enzymes and antibiotics.