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Synthetic biology approach for mining new enzymes from unculturable microorganisms

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Synthetic biology approach for mining new enzymes from unculturable microorganisms. / Wang, Yun; Zhang, Dayi; Huang, Wei E.

In: Luminescence, Vol. 25, No. 2, 04.2010, p. 104.

Research output: Contribution to Journal/MagazineMeeting abstractpeer-review

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APA

Vancouver

Wang Y, Zhang D, Huang WE. Synthetic biology approach for mining new enzymes from unculturable microorganisms. Luminescence. 2010 Apr;25(2):104. doi: 10.1002/bio.1211

Author

Wang, Yun ; Zhang, Dayi ; Huang, Wei E. / Synthetic biology approach for mining new enzymes from unculturable microorganisms. In: Luminescence. 2010 ; Vol. 25, No. 2. pp. 104.

Bibtex

@article{066e3f6db4bc447f8bf17582dcf9bad3,
title = "Synthetic biology approach for mining new enzymes from unculturable microorganisms",
abstract = "Synthetic biology is aimed to design and rewire existing and new biological parts, devices, and systems for useful purposes. The vast majority of microbes (>99%) in natural environment are not yet culturable using traditional approaches. Those unculturable microorganisms in natural environment are an untapped gene resource which is believed to encode novel industrial catalysts and enzymes, as well as new medical compounds. We developed a novel synthetic biology approach that employs a gene trapping circuit to screen functional genes encoded with special enzymes. Using this approach, we have discovered fi ve new naphthalene and toluene dioxygenases which can be used as industrial biocatalysts such as synthesis of HIV inhibitor. This synthetic biology approach paved the way for mining the gene pool of unculturable microorganisms and searching novel enzymes and antibiotics.",
keywords = "ADP1, IN-SITU HYBRIDIZATION",
author = "Yun Wang and Dayi Zhang and Huang, {Wei E.}",
year = "2010",
month = apr,
doi = "10.1002/bio.1211",
language = "English",
volume = "25",
pages = "104",
journal = "Luminescence",
issn = "1522-7235",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Synthetic biology approach for mining new enzymes from unculturable microorganisms

AU - Wang, Yun

AU - Zhang, Dayi

AU - Huang, Wei E.

PY - 2010/4

Y1 - 2010/4

N2 - Synthetic biology is aimed to design and rewire existing and new biological parts, devices, and systems for useful purposes. The vast majority of microbes (>99%) in natural environment are not yet culturable using traditional approaches. Those unculturable microorganisms in natural environment are an untapped gene resource which is believed to encode novel industrial catalysts and enzymes, as well as new medical compounds. We developed a novel synthetic biology approach that employs a gene trapping circuit to screen functional genes encoded with special enzymes. Using this approach, we have discovered fi ve new naphthalene and toluene dioxygenases which can be used as industrial biocatalysts such as synthesis of HIV inhibitor. This synthetic biology approach paved the way for mining the gene pool of unculturable microorganisms and searching novel enzymes and antibiotics.

AB - Synthetic biology is aimed to design and rewire existing and new biological parts, devices, and systems for useful purposes. The vast majority of microbes (>99%) in natural environment are not yet culturable using traditional approaches. Those unculturable microorganisms in natural environment are an untapped gene resource which is believed to encode novel industrial catalysts and enzymes, as well as new medical compounds. We developed a novel synthetic biology approach that employs a gene trapping circuit to screen functional genes encoded with special enzymes. Using this approach, we have discovered fi ve new naphthalene and toluene dioxygenases which can be used as industrial biocatalysts such as synthesis of HIV inhibitor. This synthetic biology approach paved the way for mining the gene pool of unculturable microorganisms and searching novel enzymes and antibiotics.

KW - ADP1

KW - IN-SITU HYBRIDIZATION

U2 - 10.1002/bio.1211

DO - 10.1002/bio.1211

M3 - Meeting abstract

VL - 25

SP - 104

JO - Luminescence

JF - Luminescence

SN - 1522-7235

IS - 2

ER -