Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal.
AU - Mustacchi, Roberta
AU - Knowles, Christopher J.
AU - Li, Hong
AU - Dalrymple, Ian
AU - Sunderland, Garry
AU - Skibar, Wolfgang
AU - Jackman, Simon A.
PY - 2005/8/20
Y1 - 2005/8/20
N2 - The nitrilase of Rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. Application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. Cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g dcw with free cells to 0.040 mmol/min/g dcw with immobilised cells. When the electric current was applied, the biotransformation rate increased to 0.047 mmol/min/g dcw and product recovery increased from 19% to 79%.
AB - The nitrilase of Rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. Application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. Cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g dcw with free cells to 0.040 mmol/min/g dcw with immobilised cells. When the electric current was applied, the biotransformation rate increased to 0.047 mmol/min/g dcw and product recovery increased from 19% to 79%.
KW - immobilised biocatalysis • alginate • nitrilase • Rhodococcus rhodochrous • electrokinetics • product recovery
U2 - 10.1002/bit.20522
DO - 10.1002/bit.20522
M3 - Journal article
VL - 91
SP - 436
EP - 440
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 1097-0290
IS - 4
ER -