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The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal. / Mustacchi, Roberta; Knowles, Christopher J.; Li, Hong et al.
In: Biotechnology and Bioengineering, Vol. 91, No. 4, 20.08.2005, p. 436-440.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Mustacchi, R, Knowles, CJ, Li, H, Dalrymple, I, Sunderland, G, Skibar, W & Jackman, SA 2005, 'The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal.', Biotechnology and Bioengineering, vol. 91, no. 4, pp. 436-440. https://doi.org/10.1002/bit.20522

APA

Mustacchi, R., Knowles, C. J., Li, H., Dalrymple, I., Sunderland, G., Skibar, W., & Jackman, S. A. (2005). The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal. Biotechnology and Bioengineering, 91(4), 436-440. https://doi.org/10.1002/bit.20522

Vancouver

Mustacchi R, Knowles CJ, Li H, Dalrymple I, Sunderland G, Skibar W et al. The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal. Biotechnology and Bioengineering. 2005 Aug 20;91(4):436-440. doi: 10.1002/bit.20522

Author

Mustacchi, Roberta ; Knowles, Christopher J. ; Li, Hong et al. / The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal. In: Biotechnology and Bioengineering. 2005 ; Vol. 91, No. 4. pp. 436-440.

Bibtex

@article{32c528663c494b4ca417bc88c2dcc007,
title = "The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal.",
abstract = "The nitrilase of Rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. Application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. Cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g dcw with free cells to 0.040 mmol/min/g dcw with immobilised cells. When the electric current was applied, the biotransformation rate increased to 0.047 mmol/min/g dcw and product recovery increased from 19% to 79%.",
keywords = "immobilised biocatalysis • alginate • nitrilase • Rhodococcus rhodochrous • electrokinetics • product recovery",
author = "Roberta Mustacchi and Knowles, {Christopher J.} and Hong Li and Ian Dalrymple and Garry Sunderland and Wolfgang Skibar and Jackman, {Simon A.}",
year = "2005",
month = aug,
day = "20",
doi = "10.1002/bit.20522",
language = "English",
volume = "91",
pages = "436--440",
journal = "Biotechnology and Bioengineering",
issn = "1097-0290",
publisher = "Wiley-VCH Verlag",
number = "4",

}

RIS

TY - JOUR

T1 - The effect of whole cell immobilisation on the biotransformation of benzonitrile and the use of direct electric current for enhanced product removal.

AU - Mustacchi, Roberta

AU - Knowles, Christopher J.

AU - Li, Hong

AU - Dalrymple, Ian

AU - Sunderland, Garry

AU - Skibar, Wolfgang

AU - Jackman, Simon A.

PY - 2005/8/20

Y1 - 2005/8/20

N2 - The nitrilase of Rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. Application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. Cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g dcw with free cells to 0.040 mmol/min/g dcw with immobilised cells. When the electric current was applied, the biotransformation rate increased to 0.047 mmol/min/g dcw and product recovery increased from 19% to 79%.

AB - The nitrilase of Rhodococcus rhodochrous performs a one-step biotransformation of nitriles to their corresponding carboxylic acids. Application of a direct electric current moves the charged carboxylic acid towards an anode, across an anion exchange membrane, into a separate compartment. Cells encapsulated within alginate beads (2.9 mm diameter) for protection against the current biotransformed benzonitrile to benzoic acid with a 26% reduction in the biotransformation rate, from 0.054 mmol/min/g dcw with free cells to 0.040 mmol/min/g dcw with immobilised cells. When the electric current was applied, the biotransformation rate increased to 0.047 mmol/min/g dcw and product recovery increased from 19% to 79%.

KW - immobilised biocatalysis • alginate • nitrilase • Rhodococcus rhodochrous • electrokinetics • product recovery

U2 - 10.1002/bit.20522

DO - 10.1002/bit.20522

M3 - Journal article

VL - 91

SP - 436

EP - 440

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 1097-0290

IS - 4

ER -