The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Standard

The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. / Wilson, Gayle M.; Fielding, Andrew B.; Simon, Glenn C. et al.
In: Molecular Biology of the Cell, Vol. 16, No. 2, 02.2005, p. 849-860.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Wilson, GM, Fielding, AB, Simon, GC, Yu, X, Andrews, PD, Hames, RS, Frey, AM, Peden, AA, Gould, GW & Prekeris, R 2005, 'The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis', Molecular Biology of the Cell, vol. 16, no. 2, pp. 849-860. https://doi.org/10.1091/mbc.E04-10-0927

APA

Wilson, G. M., Fielding, A. B., Simon, G. C., Yu, X., Andrews, P. D., Hames, R. S., Frey, A. M., Peden, A. A., Gould, G. W., & Prekeris, R. (2005). The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Molecular Biology of the Cell, 16(2), 849-860. https://doi.org/10.1091/mbc.E04-10-0927

Vancouver

Wilson GM, Fielding AB, Simon GC, Yu X, Andrews PD, Hames RS et al. The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Molecular Biology of the Cell. 2005 Feb;16(2):849-860. doi: 10.1091/mbc.E04-10-0927

Author

Wilson, Gayle M. ; Fielding, Andrew B. ; Simon, Glenn C. et al. / The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 2. pp. 849-860.

Bibtex

@article{e97d27087ade4e879867472b5ffbf352,

title = "The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis",

abstract = "An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.",

keywords = "Animals, Carrier Proteins, Cell Cycle, Cell Membrane, Cytokinesis, DNA-Binding Proteins, Endosomes, Flow Cytometry, Fluorescence Resonance Energy Transfer, HeLa Cells, Humans, I-kappa B Kinase, Mice, Microscopy, Confocal, Microscopy, Video, NIH 3T3 Cells, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference, Time Factors, Transcription Factors, Upstream Stimulatory Factors, rab GTP-Binding Proteins, Journal Article, Research Support, Non-U.S. Gov't",

author = "Wilson, {Gayle M.} and Fielding, {Andrew B.} and Simon, {Glenn C.} and Xinzi Yu and Andrews, {Paul D.} and Hames, {Rebecca S.} and Frey, {Andrew M.} and Peden, {Andrew A.} and Gould, {Gwyn W.} and Rytis Prekeris",

year = "2005",

month = feb,

doi = "10.1091/mbc.E04-10-0927",

language = "English",

volume = "16",

pages = "849--860",

journal = "Molecular Biology of the Cell",

issn = "1059-1524",

publisher = "American Society for Cell Biology",

number = "2",

}

RIS

TY - JOUR

T1 - The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis

AU - Wilson, Gayle M.

AU - Fielding, Andrew B.

AU - Simon, Glenn C.

AU - Yu, Xinzi

AU - Andrews, Paul D.

AU - Hames, Rebecca S.

AU - Frey, Andrew M.

AU - Peden, Andrew A.

AU - Gould, Gwyn W.

AU - Prekeris, Rytis

PY - 2005/2

Y1 - 2005/2

N2 - An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.

AB - An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.

KW - Animals

KW - Carrier Proteins

KW - Cell Cycle

KW - Cell Membrane

KW - Cytokinesis

KW - DNA-Binding Proteins

KW - Endosomes

KW - Flow Cytometry

KW - Fluorescence Resonance Energy Transfer

KW - HeLa Cells

KW - Humans

KW - I-kappa B Kinase

KW - Mice

KW - Microscopy, Confocal

KW - Microscopy, Video

KW - NIH 3T3 Cells

KW - Protein Transport

KW - Protein-Serine-Threonine Kinases

KW - RNA Interference

KW - Time Factors

KW - Transcription Factors

KW - Upstream Stimulatory Factors

KW - rab GTP-Binding Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1091/mbc.E04-10-0927

DO - 10.1091/mbc.E04-10-0927

M3 - Journal article

C2 - 15601896

VL - 16

SP - 849

EP - 860

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 2

ER -

Research

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