Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis
AU - Wilson, Gayle M.
AU - Fielding, Andrew B.
AU - Simon, Glenn C.
AU - Yu, Xinzi
AU - Andrews, Paul D.
AU - Hames, Rebecca S.
AU - Frey, Andrew M.
AU - Peden, Andrew A.
AU - Gould, Gwyn W.
AU - Prekeris, Rytis
PY - 2005/2
Y1 - 2005/2
N2 - An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.
AB - An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission.
KW - Animals
KW - Carrier Proteins
KW - Cell Cycle
KW - Cell Membrane
KW - Cytokinesis
KW - DNA-Binding Proteins
KW - Endosomes
KW - Flow Cytometry
KW - Fluorescence Resonance Energy Transfer
KW - HeLa Cells
KW - Humans
KW - I-kappa B Kinase
KW - Mice
KW - Microscopy, Confocal
KW - Microscopy, Video
KW - NIH 3T3 Cells
KW - Protein Transport
KW - Protein-Serine-Threonine Kinases
KW - RNA Interference
KW - Time Factors
KW - Transcription Factors
KW - Upstream Stimulatory Factors
KW - rab GTP-Binding Proteins
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1091/mbc.E04-10-0927
DO - 10.1091/mbc.E04-10-0927
M3 - Journal article
C2 - 15601896
VL - 16
SP - 849
EP - 860
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 2
ER -