Home > Research > Publications & Outputs > The role of surface glycoconjugates in Leishman...

Electronic data

  • S0031182013000358a

    Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, © 2013 Cambridge University Press.

    Final published version, 126 KB, PDF document

Links

Text available via DOI:

View graph of relations

The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies

Research output: Contribution to journalJournal article

Published
  • Lucie Jecna
  • Anna Dostalova
  • Ray Wilson
  • Veronika Seblova
  • Kwang-Poo Chang
  • Paul A. Bates
  • Petr Volf
Close
<mark>Journal publication date</mark>07/2013
<mark>Journal</mark>Parasitology
Issue number8
Volume140
Number of pages7
Pages (from-to)1026-1032
Publication StatusPublished
Early online date23/04/13
<mark>Original language</mark>English

Abstract

SUMMARY Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.

Bibliographic note

http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, © 2013 Cambridge University Press.