Home > Research > Publications & Outputs > The role of surface glycoconjugates in Leishman...

Electronic data

  • S0031182013000358a

    Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, © 2013 Cambridge University Press.

    Final published version, 126 KB, PDF document

Links

Text available via DOI:

View graph of relations

The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies. / Jecna, Lucie; Dostalova, Anna; Wilson, Ray; Seblova, Veronika; Chang, Kwang-Poo; Bates, Paul A.; Volf, Petr.

In: Parasitology, Vol. 140, No. 8, 07.2013, p. 1026-1032.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Author

Jecna, Lucie ; Dostalova, Anna ; Wilson, Ray ; Seblova, Veronika ; Chang, Kwang-Poo ; Bates, Paul A. ; Volf, Petr. / The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies. In: Parasitology. 2013 ; Vol. 140, No. 8. pp. 1026-1032.

Bibtex

@article{2593566155fb4ec1897f3de0c13f9117,
title = "The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies",
abstract = "SUMMARY Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.",
keywords = "phlebotomine sand flies, Leishmania , lipophosphoglycan , zinc protease gp63",
author = "Lucie Jecna and Anna Dostalova and Ray Wilson and Veronika Seblova and Kwang-Poo Chang and Bates, {Paul A.} and Petr Volf",
note = "http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, {\textcopyright} 2013 Cambridge University Press.",
year = "2013",
month = jul,
doi = "10.1017/S0031182013000358",
language = "English",
volume = "140",
pages = "1026--1032",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "8",

}

RIS

TY - JOUR

T1 - The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies

AU - Jecna, Lucie

AU - Dostalova, Anna

AU - Wilson, Ray

AU - Seblova, Veronika

AU - Chang, Kwang-Poo

AU - Bates, Paul A.

AU - Volf, Petr

N1 - http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 140 (8), pp 1026-1032 2013, © 2013 Cambridge University Press.

PY - 2013/7

Y1 - 2013/7

N2 - SUMMARY Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.

AB - SUMMARY Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.

KW - phlebotomine sand flies

KW - Leishmania

KW - lipophosphoglycan

KW - zinc protease gp63

U2 - 10.1017/S0031182013000358

DO - 10.1017/S0031182013000358

M3 - Journal article

C2 - 23611086

VL - 140

SP - 1026

EP - 1032

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 8

ER -