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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation
AU - Cheneler, David
AU - Bowen, James
AU - Kaklamani, Georgina
PY - 2014/9/9
Y1 - 2014/9/9
N2 - Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.
AB - Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.
KW - Bioimpedance
KW - mechanotransduction
KW - cells
KW - artificial skin
KW - tissue engineering
U2 - 10.5617/jeb.869
DO - 10.5617/jeb.869
M3 - Journal article
VL - 5
SP - 55
EP - 74
JO - Journal of Electrical Bioimpedance
JF - Journal of Electrical Bioimpedance
SN - 1891-5469
ER -