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Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface

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<mark>Journal publication date</mark>2006
<mark>Journal</mark>IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Issue number1
Volume53
Number of pages8
Pages (from-to)185-192
Publication statusPublished
Original languageEnglish

Abstract

Ultrasonic imaging of biofilms in water is difficult due to the very low contrast in acoustic impedance between the biofilm and water. In this paper, biofilms exposed to moist air are scanned through the substrate in order to obtain echoes from the biofilm/air interface. A 50 MHz scanning system was used to scan 1 mm x 1 mm areas of biofilms in a 10 microm grid pattern. Two fast Fourier transform (FFT) based methods for enhancement of the film thickness measurement resolution are compared. Using these techniques, the surface topography of biofilms with thickness less than the acoustic wavelength can be imaged.