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Ultrasonic imaging of biofilms utilizing echoes from the biofilm/air interface

Research output: Contribution to Journal/MagazineJournal articlepeer-review

<mark>Journal publication date</mark>2006
<mark>Journal</mark>IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Issue number1
Number of pages8
Pages (from-to)185-192
Publication StatusPublished
<mark>Original language</mark>English


Ultrasonic imaging of biofilms in water is difficult due to the very low contrast in acoustic impedance between the biofilm and water. In this paper, biofilms exposed to moist air are scanned through the substrate in order to obtain echoes from the biofilm/air interface. A 50 MHz scanning system was used to scan 1 mm x 1 mm areas of biofilms in a 10 microm grid pattern. Two fast Fourier transform (FFT) based methods for enhancement of the film thickness measurement resolution are compared. Using these techniques, the surface topography of biofilms with thickness less than the acoustic wavelength can be imaged.