This dataset contains high-resolution (10-minute interval) data for nitrate (concentration and load), precipitation and discharge at four measurement stations (NF, SHA, TTP, OUT) in the South West Mau, Kenya, as well as trace element data and stable isotopes of water for rainfall and stream water samples. The data was used to investigate the influence of land use on hydrological processes through time series analysis (Jacobs et al. 2018a), end member mixing analysis and estimation of the young water fraction (Jacobs et al. 2018b).