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Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025in the human gut - Supplementary Table

Dataset

  • Xin Qian (Creator)
  • Peijun Tian (Creator)
  • Guopeng Lin (Creator)
  • Xinglong Xu (Creator)
  • Gang Wang (Creator)
  • Hao ZhangYunnan Academy of Agricultural Sciences, Jilin University, Fudan Univ, Fudan University, Sch Informat Sci & Technol, Dalian University of Technology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China., Zhongnan Hospital of Wuhan University, Ocean University of China, Changhai Hospital, Second Affiliated Hospital of Nanjing Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College, Second Affiliated Hospital of Harbin Medical University, Univ Arizona, University of Arizona, Chongqing Medical University, Shanghai Children's Medical Center, Guangdong Pharmaceutical University, Jinan Univ, Jinan University, Coll Pharm, Guangdong Prov Key Lab Pharmacodynam Constituents, Northwestern Polytechnical University Xian, Nanyang Technol Univ, Nanyang Technological University, Nanyang Technological University & National Institute of Education (NIE) Singapore, Sch Mat Sci & Engn, Hubei Academy of Agricultural Sciences, South China University of Technology, Harvard Univ, Harvard University, Dept Phys, Shandong University, Qingdao, Shandong 266237, China, Huazhong University of Science and Technology, Univ Victoria, University of Victoria, Dept Phys & Astron, McGill Univ, McGill University, McGill Sch Environm, Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China, Ruian People's Hospital, First Affiliated Hospital of Harbin Medical University, Shanghai Stomatological Hospital, Arthritis Research Canada Vancouver British Columbia Canada; Department of Rehabilitation Medicine, West China Hospital Sichuan University Chengdu Sichuan China; Rehabilitation Medicine Key Laboratory of Sichuan Province, West China Hospital Sichuan University Chengdu Sichuan China, Guangdong University of Petrochemical Technology, Henan Normal University, Cornell Univ, Cornell University, Kavli Inst Cornell, Zhejiang Univ, Zhejiang University, Inst Informat & Commun Engn, Shanghai Jiao Tong Univ, Shanghai Jiao Tong University, Sch Mech Engn, Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China., Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Urology Department The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University Qingdao Shandong China, Univ Chinese Acad Sci, Chinese Academy of Sciences, University of Chinese Academy of Sciences, CAS, Jiangsu Province Hospital, Guangzhou & Chinese Academy of Sciences, Heilongjiang Provincial Hospital, The First Affiliated Hospital, Dalian Medical University, Dalian, China, Nanchang University, Nanjing Medical University, Jiangnan University (Creator)
  • Wei Chen (Creator)

Description

Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.
Date made available2023
PublisherFuture Science Group

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