We conducted coordinated measurements of realised and potential soil net Nmin, and assessed water holding capacity, bulk density, C and N content, texture, pH, pore space, microbial biomass, and archaeal (AOA) and bacterial (AOB) ammonia oxidiser abundance using identical materials and methods across 30 grasslands on six continents. The sites covered a globally relevant range of climatic and edaphic conditions. Climate data was obtained from worldclim - Global climate data https://www.worldclim.org/