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13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. / Whitby, C. B.; Hall, G.; Pickup, R. et al.
In: Letters in Applied Microbiology, Vol. 32, No. 6, 01.06.2001, p. 398-401.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Whitby, CB, Hall, G, Pickup, R, Saunders, JR, Ineson, P, Parekh, NR & McCarthy, A 2001, '13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations', Letters in Applied Microbiology, vol. 32, no. 6, pp. 398-401. https://doi.org/10.1046/j.1472-765X.2001.00930.x

APA

Whitby, C. B., Hall, G., Pickup, R., Saunders, J. R., Ineson, P., Parekh, N. R., & McCarthy, A. (2001). 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. Letters in Applied Microbiology, 32(6), 398-401. https://doi.org/10.1046/j.1472-765X.2001.00930.x

Vancouver

Whitby CB, Hall G, Pickup R, Saunders JR, Ineson P, Parekh NR et al. 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. Letters in Applied Microbiology. 2001 Jun 1;32(6):398-401. doi: 10.1046/j.1472-765X.2001.00930.x

Author

Whitby, C. B. ; Hall, G. ; Pickup, R. et al. / 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. In: Letters in Applied Microbiology. 2001 ; Vol. 32, No. 6. pp. 398-401.

Bibtex

@article{3ac6734978c44afd92a043e67ecff701,
title = "13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations",
abstract = "Aims: To identify active CO2-assimilating species of ammonia-oxidizing bacteria in fresh water sediment. Methods and Results: Enrichment cultures were incubated in the presence of 13C labelled CO2, and 13C-DNA successfully resolved from 12C-DNA by caesium chloride density gradient ultracentrifugation of DNA extracts. Ammonia-oxidizer DNA recovered from these gradients was amplified and characterised by Temporal Temperature Gradient Gel Electrophoresis (TTGE), with confirmatory sequence analysis to identify the metabolically active components of the population. Conclusions: The 12C-DNA fraction was dominated by nitrosospiras, in contrast to the 13C-DNA fraction which was largely nitrosomonad DNA, in support of the hypothesis that nitrosomonads out-compete nitrosospiras in laboratory culture. Significance and Impact of the Study: The use of stable isotype incorporation into ammonia-oxidizer DNA could therefore circumvent the problems associated with RNA detection to identify metabolically active species in situ.",
author = "Whitby, {C. B.} and G. Hall and R. Pickup and Saunders, {J. R.} and P. Ineson and Parekh, {N. R.} and A. McCarthy",
year = "2001",
month = jun,
day = "1",
doi = "10.1046/j.1472-765X.2001.00930.x",
language = "English",
volume = "32",
pages = "398--401",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations

AU - Whitby, C. B.

AU - Hall, G.

AU - Pickup, R.

AU - Saunders, J. R.

AU - Ineson, P.

AU - Parekh, N. R.

AU - McCarthy, A.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Aims: To identify active CO2-assimilating species of ammonia-oxidizing bacteria in fresh water sediment. Methods and Results: Enrichment cultures were incubated in the presence of 13C labelled CO2, and 13C-DNA successfully resolved from 12C-DNA by caesium chloride density gradient ultracentrifugation of DNA extracts. Ammonia-oxidizer DNA recovered from these gradients was amplified and characterised by Temporal Temperature Gradient Gel Electrophoresis (TTGE), with confirmatory sequence analysis to identify the metabolically active components of the population. Conclusions: The 12C-DNA fraction was dominated by nitrosospiras, in contrast to the 13C-DNA fraction which was largely nitrosomonad DNA, in support of the hypothesis that nitrosomonads out-compete nitrosospiras in laboratory culture. Significance and Impact of the Study: The use of stable isotype incorporation into ammonia-oxidizer DNA could therefore circumvent the problems associated with RNA detection to identify metabolically active species in situ.

AB - Aims: To identify active CO2-assimilating species of ammonia-oxidizing bacteria in fresh water sediment. Methods and Results: Enrichment cultures were incubated in the presence of 13C labelled CO2, and 13C-DNA successfully resolved from 12C-DNA by caesium chloride density gradient ultracentrifugation of DNA extracts. Ammonia-oxidizer DNA recovered from these gradients was amplified and characterised by Temporal Temperature Gradient Gel Electrophoresis (TTGE), with confirmatory sequence analysis to identify the metabolically active components of the population. Conclusions: The 12C-DNA fraction was dominated by nitrosospiras, in contrast to the 13C-DNA fraction which was largely nitrosomonad DNA, in support of the hypothesis that nitrosomonads out-compete nitrosospiras in laboratory culture. Significance and Impact of the Study: The use of stable isotype incorporation into ammonia-oxidizer DNA could therefore circumvent the problems associated with RNA detection to identify metabolically active species in situ.

U2 - 10.1046/j.1472-765X.2001.00930.x

DO - 10.1046/j.1472-765X.2001.00930.x

M3 - Journal article

C2 - 11412351

AN - SCOPUS:0034995535

VL - 32

SP - 398

EP - 401

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -