Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - 14-3-3 proteins intact with a 13-lipoxygenase, but not with a 9-lipoxygenase.
AU - Holtman, Wessel L.
AU - Roberts, Michael R.
AU - Oppedijk, Berry J.
AU - Testerink, Christa
AU - Van Zeijl, Mieke J.
AU - Wang, Mei
PY - 2000/5/26
Y1 - 2000/5/26
N2 - Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.
AB - Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.
KW - 14-3-3 protein
KW - Lipoxygenase
KW - Interaction
U2 - 10.1016/S0014-5793(00)01575-1
DO - 10.1016/S0014-5793(00)01575-1
M3 - Journal article
VL - 474
SP - 48
EP - 52
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -