2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.).

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https://doi.org/10.1042/bj3160389
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2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.). / Andralojc, P. John; Ryan, Jane E.; Parry, M. A. J. et al.
In: The Biochemical journal, Vol. 316, No. 2, 06.1996, p. 389-393.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{25ea9756a14e4d199c42f29115028344,

title = "2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.).",

abstract = "Transfer of the phosphate group of 2-carboxy-D-arabinitol 1-phosphate (CA1P) to 14C-labelled 2-carboxy-D-arabinitol (CA) was catalysed by extracts from leaves of Phaseolus vulgaris. This phosphotransferase activity co-purified with CA1P phosphatase, described previously. This activity was increased, up to 16-fold, by addition of bicarbonate ions at pH 9-10, suggesting rate enhancement by enzyme carbamylation. A Vmax of 1.5 μmol/min per mg of protein and a Km (for CA) of 1.8 mM were estimated for the exchange reaction, with the purified phosphatase. 2-Carboxy-D-arabinitol 1,5-bisphosphate and 2-carboxy-D-ribitol 1,5-bisphosphate, but not D-ribulose 1,5-bisphosphate, could replace CA1P as phosphate donor to [14C]CA.",

author = "Andralojc, {P. John} and Ryan, {Jane E.} and Parry, {M. A. J.} and Keys, {Alfred J.}",

year = "1996",

month = jun,

doi = "10.1042/bj3160389",

language = "English",

volume = "316",

pages = "389--393",

journal = "The Biochemical journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "2",

}

RIS

TY - JOUR

T1 - 2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.).

AU - Andralojc, P. John

AU - Ryan, Jane E.

AU - Parry, M. A. J.

AU - Keys, Alfred J.

PY - 1996/6

Y1 - 1996/6

N2 - Transfer of the phosphate group of 2-carboxy-D-arabinitol 1-phosphate (CA1P) to 14C-labelled 2-carboxy-D-arabinitol (CA) was catalysed by extracts from leaves of Phaseolus vulgaris. This phosphotransferase activity co-purified with CA1P phosphatase, described previously. This activity was increased, up to 16-fold, by addition of bicarbonate ions at pH 9-10, suggesting rate enhancement by enzyme carbamylation. A Vmax of 1.5 μmol/min per mg of protein and a Km (for CA) of 1.8 mM were estimated for the exchange reaction, with the purified phosphatase. 2-Carboxy-D-arabinitol 1,5-bisphosphate and 2-carboxy-D-ribitol 1,5-bisphosphate, but not D-ribulose 1,5-bisphosphate, could replace CA1P as phosphate donor to [14C]CA.

AB - Transfer of the phosphate group of 2-carboxy-D-arabinitol 1-phosphate (CA1P) to 14C-labelled 2-carboxy-D-arabinitol (CA) was catalysed by extracts from leaves of Phaseolus vulgaris. This phosphotransferase activity co-purified with CA1P phosphatase, described previously. This activity was increased, up to 16-fold, by addition of bicarbonate ions at pH 9-10, suggesting rate enhancement by enzyme carbamylation. A Vmax of 1.5 μmol/min per mg of protein and a Km (for CA) of 1.8 mM were estimated for the exchange reaction, with the purified phosphatase. 2-Carboxy-D-arabinitol 1,5-bisphosphate and 2-carboxy-D-ribitol 1,5-bisphosphate, but not D-ribulose 1,5-bisphosphate, could replace CA1P as phosphate donor to [14C]CA.

U2 - 10.1042/bj3160389

DO - 10.1042/bj3160389

M3 - Journal article

C2 - 8687378

AN - SCOPUS:0030008537

VL - 316

SP - 389

EP - 393

JO - The Biochemical journal

JF - The Biochemical journal

SN - 0264-6021

IS - 2

ER -

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