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  • Hardy Samadikuchaksaraei - Biomacromol - 2018 - silk bilayer films for wound healing

    Rights statement: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright ©2018 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.biomac.7b01807

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3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

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3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo. / Gholipourmalekabadi, Mazaher; Seifalian, Alexander; Urbanska, Aleksandra et al.
In: Biomacromolecules, Vol. 19, No. 7, 09.07.2018, p. 2409-2422.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Gholipourmalekabadi, M, Seifalian, A, Urbanska, A, Omrani, M, Hardy, JG, Madjd, Z, Hashemi, S, Ghanbarian, H, Milan, P, Mozafari, M, Reis, R, Kundu, S & Samadikuchaksaraei, A 2018, '3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo', Biomacromolecules, vol. 19, no. 7, pp. 2409-2422. https://doi.org/10.1021/acs.biomac.7b01807

APA

Gholipourmalekabadi, M., Seifalian, A., Urbanska, A., Omrani, M., Hardy, J. G., Madjd, Z., Hashemi, S., Ghanbarian, H., Milan, P., Mozafari, M., Reis, R., Kundu, S., & Samadikuchaksaraei, A. (2018). 3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo. Biomacromolecules, 19(7), 2409-2422. https://doi.org/10.1021/acs.biomac.7b01807

Vancouver

Gholipourmalekabadi M, Seifalian A, Urbanska A, Omrani M, Hardy JG, Madjd Z et al. 3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo. Biomacromolecules. 2018 Jul 9;19(7):2409-2422. Epub 2018 Mar 12. doi: 10.1021/acs.biomac.7b01807

Author

Gholipourmalekabadi, Mazaher ; Seifalian, Alexander ; Urbanska, Aleksandra et al. / 3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo. In: Biomacromolecules. 2018 ; Vol. 19, No. 7. pp. 2409-2422.

Bibtex

@article{7d7012b47ecb4175a3d5d5455e31c6fb,
title = "3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo",
abstract = "Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds. In this report we evaluate finally about the appropriate utility of this fabricated bi-layered artificial skin in vivo with particular reference to healing and scar formation due to biochemical and biomechanical complexities of the skin. For this work. AM, AM/ESF alone or seeded with adipose tissue-derived mesenchymal stem cells (AT-MSCs) are implanted to full thickness burn wounds in mice. The healing efficacy and scar formation are evaluated at 7, 14 and 28 days post-implantation in vivo. Our data reveal that ESF accelerates wound healing process through early recruitment of inflammatory cells such as macrophages into the defective site, as well as up-regulation of angiogenic factors from the AT-MSCs and facilitation of remodeling phase. In vivo application of the prepared AM/ESF membrane seeded with the AT-MSCs reduces significantly the post-burn scars. The in vivo data suggest that the potential applications of the AM/ESF bi-layered artificial skin may be considered as a clinically translational product with stem cells to guide scarless healing of sever burn injuries. ",
keywords = "silk, wound healing, biomaterials, regenerative medicine, materials science",
author = "Mazaher Gholipourmalekabadi and Alexander Seifalian and Aleksandra Urbanska and Mir Omrani and Hardy, {John George} and Zahra Madjd and Seyed Hashemi and Hossein Ghanbarian and Peiman Milan and Masoud Mozafari and Rui Reis and Subhas Kundu and Ali Samadikuchaksaraei",
note = "This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright {\textcopyright}2018 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.biomac.7b01807",
year = "2018",
month = jul,
day = "9",
doi = "10.1021/acs.biomac.7b01807",
language = "English",
volume = "19",
pages = "2409--2422",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "7",

}

RIS

TY - JOUR

T1 - 3D protein-based bilayer artificial skin for guided scarless healing of full-thickness burn wounds in vivo

AU - Gholipourmalekabadi, Mazaher

AU - Seifalian, Alexander

AU - Urbanska, Aleksandra

AU - Omrani, Mir

AU - Hardy, John George

AU - Madjd, Zahra

AU - Hashemi, Seyed

AU - Ghanbarian, Hossein

AU - Milan, Peiman

AU - Mozafari, Masoud

AU - Reis, Rui

AU - Kundu, Subhas

AU - Samadikuchaksaraei, Ali

N1 - This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright ©2018 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.biomac.7b01807

PY - 2018/7/9

Y1 - 2018/7/9

N2 - Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds. In this report we evaluate finally about the appropriate utility of this fabricated bi-layered artificial skin in vivo with particular reference to healing and scar formation due to biochemical and biomechanical complexities of the skin. For this work. AM, AM/ESF alone or seeded with adipose tissue-derived mesenchymal stem cells (AT-MSCs) are implanted to full thickness burn wounds in mice. The healing efficacy and scar formation are evaluated at 7, 14 and 28 days post-implantation in vivo. Our data reveal that ESF accelerates wound healing process through early recruitment of inflammatory cells such as macrophages into the defective site, as well as up-regulation of angiogenic factors from the AT-MSCs and facilitation of remodeling phase. In vivo application of the prepared AM/ESF membrane seeded with the AT-MSCs reduces significantly the post-burn scars. The in vivo data suggest that the potential applications of the AM/ESF bi-layered artificial skin may be considered as a clinically translational product with stem cells to guide scarless healing of sever burn injuries.

AB - Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds. In this report we evaluate finally about the appropriate utility of this fabricated bi-layered artificial skin in vivo with particular reference to healing and scar formation due to biochemical and biomechanical complexities of the skin. For this work. AM, AM/ESF alone or seeded with adipose tissue-derived mesenchymal stem cells (AT-MSCs) are implanted to full thickness burn wounds in mice. The healing efficacy and scar formation are evaluated at 7, 14 and 28 days post-implantation in vivo. Our data reveal that ESF accelerates wound healing process through early recruitment of inflammatory cells such as macrophages into the defective site, as well as up-regulation of angiogenic factors from the AT-MSCs and facilitation of remodeling phase. In vivo application of the prepared AM/ESF membrane seeded with the AT-MSCs reduces significantly the post-burn scars. The in vivo data suggest that the potential applications of the AM/ESF bi-layered artificial skin may be considered as a clinically translational product with stem cells to guide scarless healing of sever burn injuries.

KW - silk

KW - wound healing

KW - biomaterials

KW - regenerative medicine

KW - materials science

U2 - 10.1021/acs.biomac.7b01807

DO - 10.1021/acs.biomac.7b01807

M3 - Journal article

VL - 19

SP - 2409

EP - 2422

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 7

ER -