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A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor.

Research output: Contribution to Journal/MagazineJournal article

  • I. Kurucz
  • C. R. Jost
  • A. J. T. George
  • S. M. Andrew
  • D. M. Segal
<mark>Journal publication date</mark>1993
<mark>Journal</mark>Proceedings of the National Academy of Sciences of the United States of America
Issue number9
Number of pages5
Pages (from-to)3830-3834
Publication StatusPublished
<mark>Original language</mark>English


A single-chain Fv construct of the 2B4 T-cell receptor has been made and expressed in Escherichia coli as bacterial inclusion bodies. After solubilization in 6 M guanidine hydrochloride and formation of mixed disulfides with glutathione, the protein was refolded by diluting out the denaturant and allowing intramolecular disulfide bridges to form by disulfide exchange. Approximately 65-100 mg of refolded protein was obtained from 1 liter of bacterial culture, an appreciable fraction of which was monomeric in nondenaturing solvents. This protein bound to three monoclonal antibodies specific for allotypic or idiotypic determinants on the native 2B4 variable region but did not bind several other anti-T-cell-receptor monoclonal antibodies that lacked such specificity. These experiments show that T-cell-receptor variable regions, like the V regions of antibodies, can form a well-behaved single-chain Fv molecule and provide large amounts of recombinant single-chain Fv T-cell receptor that can be used to study T-cell function.