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A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil.

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A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil. / Martin, Francis L.; Piearce, Trevor G.; Hewer, Alan et al.

In: Environmental Pollution, Vol. 138, No. 2, 11.2005, p. 307-315.

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@article{bc94f7f25bdb41d59d8b5814acb05c54,
title = "A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil.",
abstract = "There is a need to develop risk biomarkers during the remediation of contaminated land. We employed the earthworm, Aporrectodea longa (Ude), to determine whether genotoxicity measures could be applied to this organism's intestinal tissues. Earthworms were added, for 24 h or 7 days, to soil samples spiked with benzo[a]pyrene (B[a]P) and/or lindane. After exposure, intestinal tissues (crop/gizzard or intestine) were removed prior to the measurement in disaggregated cells of DNA single-strand breaks (SSBs) by the alkaline comet assay. Damage was quantified by comet tail length (CTL, μm). B[a]P 24-h exposure induced dose-related increases (P<0.0001) in SSBs. Earthworm intestine was significantly (P<0.0001) more susceptible than crop/gizzard to B[a]P and/or lindane. However, both tissues appeared to acquire resistance following 7-day exposure. B[a]P-DNA adducts, measured by 32P-postlabelling, showed a two-adduct-spot pattern. This preliminary investigation suggests that earthworm tissues may be incorporated into genotoxicity assays to facilitate hazard identification within terrestrial ecosystems.",
keywords = "Adducts, Benzo[a]pyrene, Contaminated soil, Comet assay, DNA single-strand breaks, Earthworms",
author = "Martin, {Francis L.} and Piearce, {Trevor G.} and Alan Hewer and Phillips, {David H.} and Semple, {Kirk T.}",
year = "2005",
month = nov,
doi = "10.1016/j.envpol.2005.03.012",
language = "English",
volume = "138",
pages = "307--315",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil.

AU - Martin, Francis L.

AU - Piearce, Trevor G.

AU - Hewer, Alan

AU - Phillips, David H.

AU - Semple, Kirk T.

PY - 2005/11

Y1 - 2005/11

N2 - There is a need to develop risk biomarkers during the remediation of contaminated land. We employed the earthworm, Aporrectodea longa (Ude), to determine whether genotoxicity measures could be applied to this organism's intestinal tissues. Earthworms were added, for 24 h or 7 days, to soil samples spiked with benzo[a]pyrene (B[a]P) and/or lindane. After exposure, intestinal tissues (crop/gizzard or intestine) were removed prior to the measurement in disaggregated cells of DNA single-strand breaks (SSBs) by the alkaline comet assay. Damage was quantified by comet tail length (CTL, μm). B[a]P 24-h exposure induced dose-related increases (P<0.0001) in SSBs. Earthworm intestine was significantly (P<0.0001) more susceptible than crop/gizzard to B[a]P and/or lindane. However, both tissues appeared to acquire resistance following 7-day exposure. B[a]P-DNA adducts, measured by 32P-postlabelling, showed a two-adduct-spot pattern. This preliminary investigation suggests that earthworm tissues may be incorporated into genotoxicity assays to facilitate hazard identification within terrestrial ecosystems.

AB - There is a need to develop risk biomarkers during the remediation of contaminated land. We employed the earthworm, Aporrectodea longa (Ude), to determine whether genotoxicity measures could be applied to this organism's intestinal tissues. Earthworms were added, for 24 h or 7 days, to soil samples spiked with benzo[a]pyrene (B[a]P) and/or lindane. After exposure, intestinal tissues (crop/gizzard or intestine) were removed prior to the measurement in disaggregated cells of DNA single-strand breaks (SSBs) by the alkaline comet assay. Damage was quantified by comet tail length (CTL, μm). B[a]P 24-h exposure induced dose-related increases (P<0.0001) in SSBs. Earthworm intestine was significantly (P<0.0001) more susceptible than crop/gizzard to B[a]P and/or lindane. However, both tissues appeared to acquire resistance following 7-day exposure. B[a]P-DNA adducts, measured by 32P-postlabelling, showed a two-adduct-spot pattern. This preliminary investigation suggests that earthworm tissues may be incorporated into genotoxicity assays to facilitate hazard identification within terrestrial ecosystems.

KW - Adducts

KW - Benzo[a]pyrene

KW - Contaminated soil

KW - Comet assay

KW - DNA single-strand breaks

KW - Earthworms

U2 - 10.1016/j.envpol.2005.03.012

DO - 10.1016/j.envpol.2005.03.012

M3 - Journal article

VL - 138

SP - 307

EP - 315

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - 2

ER -