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A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores

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A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores. / Foster, Gary D.; Robinson, Susan W.; Blundell, Robert P.; Roberts, Michael; Hodge, Rachel; Draper, John; Scott, Rod.

In: Plant Science, Vol. 84, No. 2, 1992, p. 187-192.

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Foster, Gary D. ; Robinson, Susan W. ; Blundell, Robert P. ; Roberts, Michael ; Hodge, Rachel ; Draper, John ; Scott, Rod. / A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores. In: Plant Science. 1992 ; Vol. 84, No. 2. pp. 187-192.

Bibtex

@article{15b21229c5914fcbb26d85159cca4996,
title = "A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores",
abstract = "The E2 cDNA was isolated by differential screening of a Brassica napus library made from anthers of 1.8–4 mm in length. Northern gel and RNA dot blot analysis revealed that the E2 cDNA hybridised to a 0.7-kb mRNA transcript in RNA extracted from anthers and extruded immature microspores. High expression is limited to the microspore development phase. In situ hybridisation indicated that E2 transcript is also expressed within the tapetal cells. The sequence of 658 nucleotides was determined from clone E2B containing one major open reading frame, encoding a putative protein of Mr 12.5 kDa. This protein is cysteine rich, encodes a potential signal sequence and shows high homology with plant phospholipid transfer proteins.",
keywords = "Brassica napus, tapetum, microspore",
author = "Foster, {Gary D.} and Robinson, {Susan W.} and Blundell, {Robert P.} and Michael Roberts and Rachel Hodge and John Draper and Rod Scott",
year = "1992",
doi = "10.1016/0168-9452(92)90133-7",
language = "English",
volume = "84",
pages = "187--192",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores

AU - Foster, Gary D.

AU - Robinson, Susan W.

AU - Blundell, Robert P.

AU - Roberts, Michael

AU - Hodge, Rachel

AU - Draper, John

AU - Scott, Rod

PY - 1992

Y1 - 1992

N2 - The E2 cDNA was isolated by differential screening of a Brassica napus library made from anthers of 1.8–4 mm in length. Northern gel and RNA dot blot analysis revealed that the E2 cDNA hybridised to a 0.7-kb mRNA transcript in RNA extracted from anthers and extruded immature microspores. High expression is limited to the microspore development phase. In situ hybridisation indicated that E2 transcript is also expressed within the tapetal cells. The sequence of 658 nucleotides was determined from clone E2B containing one major open reading frame, encoding a putative protein of Mr 12.5 kDa. This protein is cysteine rich, encodes a potential signal sequence and shows high homology with plant phospholipid transfer proteins.

AB - The E2 cDNA was isolated by differential screening of a Brassica napus library made from anthers of 1.8–4 mm in length. Northern gel and RNA dot blot analysis revealed that the E2 cDNA hybridised to a 0.7-kb mRNA transcript in RNA extracted from anthers and extruded immature microspores. High expression is limited to the microspore development phase. In situ hybridisation indicated that E2 transcript is also expressed within the tapetal cells. The sequence of 658 nucleotides was determined from clone E2B containing one major open reading frame, encoding a putative protein of Mr 12.5 kDa. This protein is cysteine rich, encodes a potential signal sequence and shows high homology with plant phospholipid transfer proteins.

KW - Brassica napus

KW - tapetum

KW - microspore

U2 - 10.1016/0168-9452(92)90133-7

DO - 10.1016/0168-9452(92)90133-7

M3 - Journal article

VL - 84

SP - 187

EP - 192

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 2

ER -