Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - A Brassica napus mRNA encoding a protein homologous to phospholipid transfer proteins, is expressed specifically in the tapetum and developing microspores
AU - Foster, Gary D.
AU - Robinson, Susan W.
AU - Blundell, Robert P.
AU - Roberts, Michael
AU - Hodge, Rachel
AU - Draper, John
AU - Scott, Rod
PY - 1992
Y1 - 1992
N2 - The E2 cDNA was isolated by differential screening of a Brassica napus library made from anthers of 1.8–4 mm in length. Northern gel and RNA dot blot analysis revealed that the E2 cDNA hybridised to a 0.7-kb mRNA transcript in RNA extracted from anthers and extruded immature microspores. High expression is limited to the microspore development phase. In situ hybridisation indicated that E2 transcript is also expressed within the tapetal cells. The sequence of 658 nucleotides was determined from clone E2B containing one major open reading frame, encoding a putative protein of Mr 12.5 kDa. This protein is cysteine rich, encodes a potential signal sequence and shows high homology with plant phospholipid transfer proteins.
AB - The E2 cDNA was isolated by differential screening of a Brassica napus library made from anthers of 1.8–4 mm in length. Northern gel and RNA dot blot analysis revealed that the E2 cDNA hybridised to a 0.7-kb mRNA transcript in RNA extracted from anthers and extruded immature microspores. High expression is limited to the microspore development phase. In situ hybridisation indicated that E2 transcript is also expressed within the tapetal cells. The sequence of 658 nucleotides was determined from clone E2B containing one major open reading frame, encoding a putative protein of Mr 12.5 kDa. This protein is cysteine rich, encodes a potential signal sequence and shows high homology with plant phospholipid transfer proteins.
KW - Brassica napus
KW - tapetum
KW - microspore
U2 - 10.1016/0168-9452(92)90133-7
DO - 10.1016/0168-9452(92)90133-7
M3 - Journal article
VL - 84
SP - 187
EP - 192
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -