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A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice

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A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. / Ford, Matthew J.; Yeyati, Patricia L.; Mali, Girish R. et al.
In: Developmental Cell, Vol. 47, No. 4, 19.11.2018, p. 509-523.e5.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ford, MJ, Yeyati, PL, Mali, GR, Keighren, MA, Waddell, SH, Mjoseng, HK, Douglas, AT, Hall, EA, Sakaue-Sawano, A, Miyawaki, A, Meehan, RR, Boulter, L, Jackson, IJ, Mill, P & Mort, RL 2018, 'A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice', Developmental Cell, vol. 47, no. 4, pp. 509-523.e5. https://doi.org/10.1016/j.devcel.2018.10.027

APA

Ford, M. J., Yeyati, P. L., Mali, G. R., Keighren, M. A., Waddell, S. H., Mjoseng, H. K., Douglas, A. T., Hall, E. A., Sakaue-Sawano, A., Miyawaki, A., Meehan, R. R., Boulter, L., Jackson, I. J., Mill, P., & Mort, R. L. (2018). A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Developmental Cell, 47(4), 509-523.e5. https://doi.org/10.1016/j.devcel.2018.10.027

Vancouver

Ford MJ, Yeyati PL, Mali GR, Keighren MA, Waddell SH, Mjoseng HK et al. A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Developmental Cell. 2018 Nov 19;47(4):509-523.e5. doi: 10.1016/j.devcel.2018.10.027

Author

Ford, Matthew J. ; Yeyati, Patricia L. ; Mali, Girish R. et al. / A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. In: Developmental Cell. 2018 ; Vol. 47, No. 4. pp. 509-523.e5.

Bibtex

@article{eeb3bc8db7d84f2c851c9b555d61aae4,
title = "A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice",
abstract = "The cilia and cell cycles are inextricably linked. Centrioles in the basal body of cilia nucleate the ciliary axoneme and sequester pericentriolar matrix (PCM) at the centrosome to organize the mitotic spindle. Cilia themselves respond to growth signals, prompting cilia resorption and cell cycle re-entry. We describe a fluorescent cilia and cell cycle biosensor allowing live imaging of cell cycle progression and cilia assembly and disassembly kinetics in cells and inducible mice. We define assembly and disassembly in relation to cell cycle stage with single-cell resolution and explore the intercellular heterogeneity in cilia kinetics. In all cells and tissues analyzed, we observed cilia that persist through the G1/S transition and into S/G2/M-phase. We conclude that persistence of cilia after the G1/S transition is a general property. This resource will shed light at an individual cell level on the interplay between the cilia and cell cycles in development, regeneration, and disease. The cilia and cell cycles are fundamental processes coupled through shared machinery. Ford et al. develop and characterize a multicistronic biosensor that can simultaneously label the cell and cilia cycles in mice, enabling live imaging studies of their kinetics.",
keywords = "biosensor, cell cycle, cilia, live imaging, organoid, reporter mouse, Rosa26",
author = "Ford, {Matthew J.} and Yeyati, {Patricia L.} and Mali, {Girish R.} and Keighren, {Margaret A.} and Waddell, {Scott H.} and Mjoseng, {Heidi K.} and Douglas, {Adam T.} and Hall, {Emma A.} and Asako Sakaue-Sawano and Atsushi Miyawaki and Meehan, {Richard R.} and Luke Boulter and Jackson, {Ian J.} and Pleasantine Mill and Mort, {Richard L.}",
year = "2018",
month = nov,
day = "19",
doi = "10.1016/j.devcel.2018.10.027",
language = "English",
volume = "47",
pages = "509--523.e5",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice

AU - Ford, Matthew J.

AU - Yeyati, Patricia L.

AU - Mali, Girish R.

AU - Keighren, Margaret A.

AU - Waddell, Scott H.

AU - Mjoseng, Heidi K.

AU - Douglas, Adam T.

AU - Hall, Emma A.

AU - Sakaue-Sawano, Asako

AU - Miyawaki, Atsushi

AU - Meehan, Richard R.

AU - Boulter, Luke

AU - Jackson, Ian J.

AU - Mill, Pleasantine

AU - Mort, Richard L.

PY - 2018/11/19

Y1 - 2018/11/19

N2 - The cilia and cell cycles are inextricably linked. Centrioles in the basal body of cilia nucleate the ciliary axoneme and sequester pericentriolar matrix (PCM) at the centrosome to organize the mitotic spindle. Cilia themselves respond to growth signals, prompting cilia resorption and cell cycle re-entry. We describe a fluorescent cilia and cell cycle biosensor allowing live imaging of cell cycle progression and cilia assembly and disassembly kinetics in cells and inducible mice. We define assembly and disassembly in relation to cell cycle stage with single-cell resolution and explore the intercellular heterogeneity in cilia kinetics. In all cells and tissues analyzed, we observed cilia that persist through the G1/S transition and into S/G2/M-phase. We conclude that persistence of cilia after the G1/S transition is a general property. This resource will shed light at an individual cell level on the interplay between the cilia and cell cycles in development, regeneration, and disease. The cilia and cell cycles are fundamental processes coupled through shared machinery. Ford et al. develop and characterize a multicistronic biosensor that can simultaneously label the cell and cilia cycles in mice, enabling live imaging studies of their kinetics.

AB - The cilia and cell cycles are inextricably linked. Centrioles in the basal body of cilia nucleate the ciliary axoneme and sequester pericentriolar matrix (PCM) at the centrosome to organize the mitotic spindle. Cilia themselves respond to growth signals, prompting cilia resorption and cell cycle re-entry. We describe a fluorescent cilia and cell cycle biosensor allowing live imaging of cell cycle progression and cilia assembly and disassembly kinetics in cells and inducible mice. We define assembly and disassembly in relation to cell cycle stage with single-cell resolution and explore the intercellular heterogeneity in cilia kinetics. In all cells and tissues analyzed, we observed cilia that persist through the G1/S transition and into S/G2/M-phase. We conclude that persistence of cilia after the G1/S transition is a general property. This resource will shed light at an individual cell level on the interplay between the cilia and cell cycles in development, regeneration, and disease. The cilia and cell cycles are fundamental processes coupled through shared machinery. Ford et al. develop and characterize a multicistronic biosensor that can simultaneously label the cell and cilia cycles in mice, enabling live imaging studies of their kinetics.

KW - biosensor

KW - cell cycle

KW - cilia

KW - live imaging

KW - organoid

KW - reporter mouse

KW - Rosa26

U2 - 10.1016/j.devcel.2018.10.027

DO - 10.1016/j.devcel.2018.10.027

M3 - Journal article

C2 - 30458140

AN - SCOPUS:85056404799

VL - 47

SP - 509-523.e5

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 4

ER -