Home > Research > Publications & Outputs > A CLC chloride channel plays an essential role ...
View graph of relations

A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations. / Odden, Delphine M.; Diatloff, Eugene; Roberts, Stephen K.
In: Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1768, No. 10, 01.10.2007, p. 2466-2477.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Odden DM, Diatloff E, Roberts SK. A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2007 Oct 1;1768(10):2466-2477. doi: 10.1016/j.bbamem.2007.05.022

Author

Odden, Delphine M. ; Diatloff, Eugene ; Roberts, Stephen K. / A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations. In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 2007 ; Vol. 1768, No. 10. pp. 2466-2477.

Bibtex

@article{b3681fae63b649808024cf624fb2a2ef,
title = "A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations.",
abstract = "A putative CLC voltage-gated anion channel gene from Aspergillus nidulans (AnCLCA) is characterised. The expression of the AnCLCA cDNA restored the iron-limited growth of the Saccharomyces cerevisiae CLC null mutant strain (gef1) suggesting that AnCLCA functions as a chloride channel. An AnCLCA conditional mutant was created and exhibited a strong and specific growth inhibition in the presence of extracellular copper concentrations > 18 μM. This sensitivity was shown to be the result of a hyper-accumulation of copper by the conditional mutant, which generates superoxide to toxic levels inhibiting the growth. Further analysis revealed that copper dependent enzymes were disrupted in the AnCLCA conditional null mutant, specifically, a reduced activity of the copper–zinc superoxide dismutase (CuZn–SOD) and enhanced activity of the cytochrome oxidase (COX). These results suggest that AnCLCA plays a key role in copper homeostasis in A. nidulans and that a malfunction of this chloride channel results in disrupted intracellular copper trafficking.",
keywords = "CLC, Chloride channel, Aspergillus nidulans, Copper homeostasis, Filamentous fungi",
author = "Odden, {Delphine M.} and Eugene Diatloff and Roberts, {Stephen K.}",
note = "RAE_import_type : Journal article RAE_uoa_type : Earth Systems and Environmental Sciences",
year = "2007",
month = oct,
day = "1",
doi = "10.1016/j.bbamem.2007.05.022",
language = "English",
volume = "1768",
pages = "2466--2477",
journal = "Biochimica et Biophysica Acta (BBA) - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - A CLC chloride channel plays an essential role in copper homeostasis in Aspergillus nidulans at increased extracellular copper concentrations.

AU - Odden, Delphine M.

AU - Diatloff, Eugene

AU - Roberts, Stephen K.

N1 - RAE_import_type : Journal article RAE_uoa_type : Earth Systems and Environmental Sciences

PY - 2007/10/1

Y1 - 2007/10/1

N2 - A putative CLC voltage-gated anion channel gene from Aspergillus nidulans (AnCLCA) is characterised. The expression of the AnCLCA cDNA restored the iron-limited growth of the Saccharomyces cerevisiae CLC null mutant strain (gef1) suggesting that AnCLCA functions as a chloride channel. An AnCLCA conditional mutant was created and exhibited a strong and specific growth inhibition in the presence of extracellular copper concentrations > 18 μM. This sensitivity was shown to be the result of a hyper-accumulation of copper by the conditional mutant, which generates superoxide to toxic levels inhibiting the growth. Further analysis revealed that copper dependent enzymes were disrupted in the AnCLCA conditional null mutant, specifically, a reduced activity of the copper–zinc superoxide dismutase (CuZn–SOD) and enhanced activity of the cytochrome oxidase (COX). These results suggest that AnCLCA plays a key role in copper homeostasis in A. nidulans and that a malfunction of this chloride channel results in disrupted intracellular copper trafficking.

AB - A putative CLC voltage-gated anion channel gene from Aspergillus nidulans (AnCLCA) is characterised. The expression of the AnCLCA cDNA restored the iron-limited growth of the Saccharomyces cerevisiae CLC null mutant strain (gef1) suggesting that AnCLCA functions as a chloride channel. An AnCLCA conditional mutant was created and exhibited a strong and specific growth inhibition in the presence of extracellular copper concentrations > 18 μM. This sensitivity was shown to be the result of a hyper-accumulation of copper by the conditional mutant, which generates superoxide to toxic levels inhibiting the growth. Further analysis revealed that copper dependent enzymes were disrupted in the AnCLCA conditional null mutant, specifically, a reduced activity of the copper–zinc superoxide dismutase (CuZn–SOD) and enhanced activity of the cytochrome oxidase (COX). These results suggest that AnCLCA plays a key role in copper homeostasis in A. nidulans and that a malfunction of this chloride channel results in disrupted intracellular copper trafficking.

KW - CLC

KW - Chloride channel

KW - Aspergillus nidulans

KW - Copper homeostasis

KW - Filamentous fungi

U2 - 10.1016/j.bbamem.2007.05.022

DO - 10.1016/j.bbamem.2007.05.022

M3 - Journal article

VL - 1768

SP - 2466

EP - 2477

JO - Biochimica et Biophysica Acta (BBA) - Biomembranes

JF - Biochimica et Biophysica Acta (BBA) - Biomembranes

SN - 0005-2736

IS - 10

ER -