Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - A Male-Predominant Cuticular Hydrocarbon, 7-Methyltricosane, is used as a Contact Pheromone in the Western Flower Thrips Frankliniella occidentalis
AU - Olaniran, Oladele A.
AU - Sudhakar, Akella V.S.
AU - Drijfhout, Falko P.
AU - Dublon, Ian A.N.
AU - Hall, David R.
AU - Hamilton, James G.C.
AU - Kirk, William D.J.
PY - 2013/4/30
Y1 - 2013/4/30
N2 - In a laboratory bioassay, adult female Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) spent more time near filter paper disks that had been exposed to adult males than near unexposed disks; this effect was not observed on disks exposed to adult females. The response could only partly be explained by the known male-produced aggregation pheromone, neryl (S)-2-methylbutanoate, suggesting the presence of an unknown male-produced compound. In gas chromatography/mass spectrometry analyses, 7-methyltricosane was detected on disks exposed to males, but not on disks exposed to females. Extracts of cuticular lipids also showed relatively large amounts of 7-methyltricosane on males, whereas only trace amounts were found on females and none on larvae. Bioassays of synthetic 7-methyltricosane showed that adults responded only after contact. The response to this compound was clearly different from that to n-tricosane or hexane-only controls. Females that contacted 7-methyltricosane on glass beads stayed in the vicinity and frequently raised the abdomen, a behavior that rejects mating attempts by males. Males stayed in the vicinity and wagged the abdomen sideways, a behavior used in fighting between males. This is the first identification of a contact pheromone in the order Thysanoptera.
AB - In a laboratory bioassay, adult female Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) spent more time near filter paper disks that had been exposed to adult males than near unexposed disks; this effect was not observed on disks exposed to adult females. The response could only partly be explained by the known male-produced aggregation pheromone, neryl (S)-2-methylbutanoate, suggesting the presence of an unknown male-produced compound. In gas chromatography/mass spectrometry analyses, 7-methyltricosane was detected on disks exposed to males, but not on disks exposed to females. Extracts of cuticular lipids also showed relatively large amounts of 7-methyltricosane on males, whereas only trace amounts were found on females and none on larvae. Bioassays of synthetic 7-methyltricosane showed that adults responded only after contact. The response to this compound was clearly different from that to n-tricosane or hexane-only controls. Females that contacted 7-methyltricosane on glass beads stayed in the vicinity and frequently raised the abdomen, a behavior that rejects mating attempts by males. Males stayed in the vicinity and wagged the abdomen sideways, a behavior used in fighting between males. This is the first identification of a contact pheromone in the order Thysanoptera.
KW - Fighting behavior
KW - Mating behavior
KW - Neryl (S)-2-methylbutanoate
KW - Thripidae
KW - Thysanoptera
KW - Video tracking
U2 - 10.1007/s10886-013-0272-5
DO - 10.1007/s10886-013-0272-5
M3 - Journal article
C2 - 23519504
AN - SCOPUS:84876157467
VL - 39
SP - 559
EP - 568
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
SN - 0098-0331
IS - 4
ER -