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A novel method for the isolation of motile bacteria using gradient culture systems

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A novel method for the isolation of motile bacteria using gradient culture systems. / Thomson, Rebecca; Pickup, Roger; Porter, Jonathan.
In: Journal of Microbiological Methods, Vol. 46, No. 2, 31.08.2001, p. 141-147.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Thomson, R, Pickup, R & Porter, J 2001, 'A novel method for the isolation of motile bacteria using gradient culture systems', Journal of Microbiological Methods, vol. 46, no. 2, pp. 141-147. https://doi.org/10.1016/S0167-7012(01)00258-5

APA

Thomson, R., Pickup, R., & Porter, J. (2001). A novel method for the isolation of motile bacteria using gradient culture systems. Journal of Microbiological Methods, 46(2), 141-147. https://doi.org/10.1016/S0167-7012(01)00258-5

Vancouver

Thomson R, Pickup R, Porter J. A novel method for the isolation of motile bacteria using gradient culture systems. Journal of Microbiological Methods. 2001 Aug 31;46(2):141-147. doi: 10.1016/S0167-7012(01)00258-5

Author

Thomson, Rebecca ; Pickup, Roger ; Porter, Jonathan. / A novel method for the isolation of motile bacteria using gradient culture systems. In: Journal of Microbiological Methods. 2001 ; Vol. 46, No. 2. pp. 141-147.

Bibtex

@article{8cb01f0fa04e4a119b657dcc04c2ce70,
title = "A novel method for the isolation of motile bacteria using gradient culture systems",
abstract = "Isolation of motile bacteria from stream water samples was achieved by using Lutrol F127 (poloxamer 407) as a gelling agent in culture media. This block copolymer has the property of repeatedly liquefying and solidifying at low and high temperatures, respectively. The ability of motile bacteria to move through liquid-state Lutrol F127 towards a higher nutrient concentration was exploited. After establishment of the nutrient gradient and inoculation, the system was cooled to liquefy the medium and kept liquid to allow motile bacteria to move. Raising the temperature allowed solidification and prevented further movement. Colonies could be easily removed. The proportion of motile isolates (determined by microscopic observation) increased from 42% in the indigenous population to 100% after isolation using the gradient system.",
keywords = "Isolation, Motile bacteria, Novel gelling agent, Nutrient gradient",
author = "Rebecca Thomson and Roger Pickup and Jonathan Porter",
note = "Funding Information: Rebecca Thomson was supported by a studentship from the Society for General Microbiology, UK. The authors wish to thank Clive Good for assistance with photography.",
year = "2001",
month = aug,
day = "31",
doi = "10.1016/S0167-7012(01)00258-5",
language = "English",
volume = "46",
pages = "141--147",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - A novel method for the isolation of motile bacteria using gradient culture systems

AU - Thomson, Rebecca

AU - Pickup, Roger

AU - Porter, Jonathan

N1 - Funding Information: Rebecca Thomson was supported by a studentship from the Society for General Microbiology, UK. The authors wish to thank Clive Good for assistance with photography.

PY - 2001/8/31

Y1 - 2001/8/31

N2 - Isolation of motile bacteria from stream water samples was achieved by using Lutrol F127 (poloxamer 407) as a gelling agent in culture media. This block copolymer has the property of repeatedly liquefying and solidifying at low and high temperatures, respectively. The ability of motile bacteria to move through liquid-state Lutrol F127 towards a higher nutrient concentration was exploited. After establishment of the nutrient gradient and inoculation, the system was cooled to liquefy the medium and kept liquid to allow motile bacteria to move. Raising the temperature allowed solidification and prevented further movement. Colonies could be easily removed. The proportion of motile isolates (determined by microscopic observation) increased from 42% in the indigenous population to 100% after isolation using the gradient system.

AB - Isolation of motile bacteria from stream water samples was achieved by using Lutrol F127 (poloxamer 407) as a gelling agent in culture media. This block copolymer has the property of repeatedly liquefying and solidifying at low and high temperatures, respectively. The ability of motile bacteria to move through liquid-state Lutrol F127 towards a higher nutrient concentration was exploited. After establishment of the nutrient gradient and inoculation, the system was cooled to liquefy the medium and kept liquid to allow motile bacteria to move. Raising the temperature allowed solidification and prevented further movement. Colonies could be easily removed. The proportion of motile isolates (determined by microscopic observation) increased from 42% in the indigenous population to 100% after isolation using the gradient system.

KW - Isolation

KW - Motile bacteria

KW - Novel gelling agent

KW - Nutrient gradient

U2 - 10.1016/S0167-7012(01)00258-5

DO - 10.1016/S0167-7012(01)00258-5

M3 - Journal article

C2 - 11412924

AN - SCOPUS:0034970665

VL - 46

SP - 141

EP - 147

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 2

ER -