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A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease

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A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease. / Campo, M. Saveria; O'neil, Brian; Grindlay, G. Joan et al.
In: Virology, Vol. 234, No. 2, 04.08.1997, p. 261-266.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Campo, MS, O'neil, B, Grindlay, GJ, Curtis, F, Knowles, G & Chandrachud, L 1997, 'A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease', Virology, vol. 234, no. 2, pp. 261-266. https://doi.org/10.1006/viro.1997.8649

APA

Campo, M. S., O'neil, B., Grindlay, G. J., Curtis, F., Knowles, G., & Chandrachud, L. (1997). A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease. Virology, 234(2), 261-266. https://doi.org/10.1006/viro.1997.8649

Vancouver

Campo MS, O'neil B, Grindlay GJ, Curtis F, Knowles G, Chandrachud L. A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease. Virology. 1997 Aug 4;234(2):261-266. doi: 10.1006/viro.1997.8649

Author

Campo, M. Saveria ; O'neil, Brian ; Grindlay, G. Joan et al. / A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease. In: Virology. 1997 ; Vol. 234, No. 2. pp. 261-266.

Bibtex

@article{468acc73b2ad4dd294dcb1392f0afebd,
title = "A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease",
abstract = "The first 200 N-terminus amino acids of the L2 capsid protein of BPV-4 (designated L2a) are an effective prophylactic vaccine against BPV-4 infection. Vaccination with L2a induces the production of virus neutralizing antibodies, and when L2a antibodies are removed from immune sera, the sera lose their neutralization activity. L2a encodes three dominant B-cell epitopes, defined as epitope 1 (amino acids 101–120), epitope 2 (aa 131–151), and epitope 3 (aa 151–170). To investigate whether any of these epitopes are responsible individually or in combination for protection against viral challenge, synthetic peptides, corresponding to the three epitopes (peptides 11, 14, and 16, respectively) and conjugated to keyhole limpet hemocyanin (KLH) were tested in vaccination challenge experiments. Calves vaccinated with the three peptides together showed no evidence of papillomavirus infection; those vaccinated with peptide 14 alone developed only early lesions which did not progress to proper papillomas and regressed rapidly; those vaccinated with peptide 11 or peptide 16 alone were not protected and proceeded to develop papillomas. Therefore the three B-cell epitopes are not conventionally “neutralizing” when presented individually, but in combination they form a complex neutralization domain, and, in particular, epitope 2, represented by peptide 14, encodes a domain responsible for disease prevention. ",
author = "Campo, {M. Saveria} and Brian O'neil and Grindlay, {G. Joan} and Fiona Curtis and Graham Knowles and Lata Chandrachud",
year = "1997",
month = aug,
day = "4",
doi = "10.1006/viro.1997.8649",
language = "English",
volume = "234",
pages = "261--266",
journal = "Virology",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - A Peptide Encoding a B-cell Epitope from the N-Terminus of the Capsid Protein L2 of Bovine Papillomavirus-4 Prevents Disease

AU - Campo, M. Saveria

AU - O'neil, Brian

AU - Grindlay, G. Joan

AU - Curtis, Fiona

AU - Knowles, Graham

AU - Chandrachud, Lata

PY - 1997/8/4

Y1 - 1997/8/4

N2 - The first 200 N-terminus amino acids of the L2 capsid protein of BPV-4 (designated L2a) are an effective prophylactic vaccine against BPV-4 infection. Vaccination with L2a induces the production of virus neutralizing antibodies, and when L2a antibodies are removed from immune sera, the sera lose their neutralization activity. L2a encodes three dominant B-cell epitopes, defined as epitope 1 (amino acids 101–120), epitope 2 (aa 131–151), and epitope 3 (aa 151–170). To investigate whether any of these epitopes are responsible individually or in combination for protection against viral challenge, synthetic peptides, corresponding to the three epitopes (peptides 11, 14, and 16, respectively) and conjugated to keyhole limpet hemocyanin (KLH) were tested in vaccination challenge experiments. Calves vaccinated with the three peptides together showed no evidence of papillomavirus infection; those vaccinated with peptide 14 alone developed only early lesions which did not progress to proper papillomas and regressed rapidly; those vaccinated with peptide 11 or peptide 16 alone were not protected and proceeded to develop papillomas. Therefore the three B-cell epitopes are not conventionally “neutralizing” when presented individually, but in combination they form a complex neutralization domain, and, in particular, epitope 2, represented by peptide 14, encodes a domain responsible for disease prevention.

AB - The first 200 N-terminus amino acids of the L2 capsid protein of BPV-4 (designated L2a) are an effective prophylactic vaccine against BPV-4 infection. Vaccination with L2a induces the production of virus neutralizing antibodies, and when L2a antibodies are removed from immune sera, the sera lose their neutralization activity. L2a encodes three dominant B-cell epitopes, defined as epitope 1 (amino acids 101–120), epitope 2 (aa 131–151), and epitope 3 (aa 151–170). To investigate whether any of these epitopes are responsible individually or in combination for protection against viral challenge, synthetic peptides, corresponding to the three epitopes (peptides 11, 14, and 16, respectively) and conjugated to keyhole limpet hemocyanin (KLH) were tested in vaccination challenge experiments. Calves vaccinated with the three peptides together showed no evidence of papillomavirus infection; those vaccinated with peptide 14 alone developed only early lesions which did not progress to proper papillomas and regressed rapidly; those vaccinated with peptide 11 or peptide 16 alone were not protected and proceeded to develop papillomas. Therefore the three B-cell epitopes are not conventionally “neutralizing” when presented individually, but in combination they form a complex neutralization domain, and, in particular, epitope 2, represented by peptide 14, encodes a domain responsible for disease prevention.

U2 - 10.1006/viro.1997.8649

DO - 10.1006/viro.1997.8649

M3 - Journal article

VL - 234

SP - 261

EP - 266

JO - Virology

JF - Virology

IS - 2

ER -