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A role for nuclear localised proteasomes in mediating auxin action.

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A role for nuclear localised proteasomes in mediating auxin action. / Bahrami, Ahmad R.; Bastow, Ruth; Rolfe, Stephen et al.
In: Plant Journal, Vol. 30, No. 6, 06.2002, p. 691-698.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Bahrami, AR, Bastow, R, Rolfe, S, Price, C & Gray, JE 2002, 'A role for nuclear localised proteasomes in mediating auxin action.', Plant Journal, vol. 30, no. 6, pp. 691-698. https://doi.org/10.1046/j.1365-313X.2002.01320.x

APA

Bahrami, A. R., Bastow, R., Rolfe, S., Price, C., & Gray, J. E. (2002). A role for nuclear localised proteasomes in mediating auxin action. Plant Journal, 30(6), 691-698. https://doi.org/10.1046/j.1365-313X.2002.01320.x

Vancouver

Bahrami AR, Bastow R, Rolfe S, Price C, Gray JE. A role for nuclear localised proteasomes in mediating auxin action. Plant Journal. 2002 Jun;30(6):691-698. doi: 10.1046/j.1365-313X.2002.01320.x

Author

Bahrami, Ahmad R. ; Bastow, Ruth ; Rolfe, Stephen et al. / A role for nuclear localised proteasomes in mediating auxin action. In: Plant Journal. 2002 ; Vol. 30, No. 6. pp. 691-698.

Bibtex

@article{d014f1e6fd2d4293a33ff094aa744f86,
title = "A role for nuclear localised proteasomes in mediating auxin action.",
abstract = "A number of important cellular events in animals and yeast are regulated by protein degradation, and it is becoming apparent that such regulated proteolysis is involved in many facets of plant physiology and development. We have investigated the role of protein degradation by proteasomes in plants using NtPSA1, a tobacco gene that is predominantly expressed in young developing tobacco tissues and has extensive homology to yeast and human α-type proteasome subunit genes. The NtPSA1 cDNA was used to complement a lethal mutation of the yeast PRC1 α subunit gene indicating that NtPSA1 encodes a functional proteasome subunit, and transient expression of an NtPSA1::GUS protein fusion in onion cells confirmed that the nuclear localisation signal that is present in the NtPSA1 peptide sequence is active in plant cells. Plants transformed with an antisense NtPSA1 gene had reduced levels of NtPSA1 mRNA and exhibited reduced apical dominance. In addition, these low NtPSA1 plants displayed several morphological defects associated with auxin resistance such as reduced stamen length, and showed increased tolerance to high concentrations of auxin. These results support a role for nuclear localised proteasomes in floral development and auxin responses.",
author = "Bahrami, {Ahmad R.} and Ruth Bastow and Stephen Rolfe and Clive Price and Gray, {Julie E.}",
year = "2002",
month = jun,
doi = "10.1046/j.1365-313X.2002.01320.x",
language = "English",
volume = "30",
pages = "691--698",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Blackwell Publishing Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - A role for nuclear localised proteasomes in mediating auxin action.

AU - Bahrami, Ahmad R.

AU - Bastow, Ruth

AU - Rolfe, Stephen

AU - Price, Clive

AU - Gray, Julie E.

PY - 2002/6

Y1 - 2002/6

N2 - A number of important cellular events in animals and yeast are regulated by protein degradation, and it is becoming apparent that such regulated proteolysis is involved in many facets of plant physiology and development. We have investigated the role of protein degradation by proteasomes in plants using NtPSA1, a tobacco gene that is predominantly expressed in young developing tobacco tissues and has extensive homology to yeast and human α-type proteasome subunit genes. The NtPSA1 cDNA was used to complement a lethal mutation of the yeast PRC1 α subunit gene indicating that NtPSA1 encodes a functional proteasome subunit, and transient expression of an NtPSA1::GUS protein fusion in onion cells confirmed that the nuclear localisation signal that is present in the NtPSA1 peptide sequence is active in plant cells. Plants transformed with an antisense NtPSA1 gene had reduced levels of NtPSA1 mRNA and exhibited reduced apical dominance. In addition, these low NtPSA1 plants displayed several morphological defects associated with auxin resistance such as reduced stamen length, and showed increased tolerance to high concentrations of auxin. These results support a role for nuclear localised proteasomes in floral development and auxin responses.

AB - A number of important cellular events in animals and yeast are regulated by protein degradation, and it is becoming apparent that such regulated proteolysis is involved in many facets of plant physiology and development. We have investigated the role of protein degradation by proteasomes in plants using NtPSA1, a tobacco gene that is predominantly expressed in young developing tobacco tissues and has extensive homology to yeast and human α-type proteasome subunit genes. The NtPSA1 cDNA was used to complement a lethal mutation of the yeast PRC1 α subunit gene indicating that NtPSA1 encodes a functional proteasome subunit, and transient expression of an NtPSA1::GUS protein fusion in onion cells confirmed that the nuclear localisation signal that is present in the NtPSA1 peptide sequence is active in plant cells. Plants transformed with an antisense NtPSA1 gene had reduced levels of NtPSA1 mRNA and exhibited reduced apical dominance. In addition, these low NtPSA1 plants displayed several morphological defects associated with auxin resistance such as reduced stamen length, and showed increased tolerance to high concentrations of auxin. These results support a role for nuclear localised proteasomes in floral development and auxin responses.

U2 - 10.1046/j.1365-313X.2002.01320.x

DO - 10.1046/j.1365-313X.2002.01320.x

M3 - Journal article

VL - 30

SP - 691

EP - 698

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 6

ER -