Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - A study on the effect of zinc oxide and zinc peroxide nanoparticles to enhance angiogenesis-pro-angiogenic grafts for tissue regeneration applications
AU - Ahtzaz, Samreen
AU - Nasir, Muhammad
AU - Shahzadi, Lubna
AU - Amir, Walija
AU - Anjum, Aneeq
AU - Arshad, Rida
AU - Iqbal, Farasat
AU - Chaudhry, Aqif Anwar
AU - Yar, Muhammad
AU - Rehman, Ihtesham Ur
PY - 2017/10/15
Y1 - 2017/10/15
N2 - Angiogenesis is a process of formation of new small blood vessels from existing vessels and is very critical for proper and rapid tissue healing. As reactive oxygen species (ROS) are known for their ability to promote angiogenesis, in this study zinc oxide (ZnO) and zinc peroxide (ZnO2) nano-particles (NP's) encapsulated chitosan (CS) and cellulose based hydrogels were synthesized. ZnO2 is comparatively stronger oxidizing agent as compared to ZnO, therefore, in this study it was hypothesized that ZnO2 would deliver better angiogenic potential than ZnO. Three types of hydrogels were prepared; control hydrogel (without nano-particles), hydrogel having ZnO NP's and hydrogel having ZnO2 NP's. The hydrogels were characterized by scanning electron microscopy (SEM) for structural morphology, Fourier transform infrared spectroscopy (FTIR) for chemical functional groups analyses and X-ray Diffraction (XRD) to investigate the crystalline or amorphous structure of NP's. The solution absorption capacity was tested in PBS and degradation was investigated in PBS, PBS/H2O2 and PBS/lysozyme solutions. The angiogenic behavior of these materials was studied in chorioallantoic membrane (CAM) assay and ZnO2 based material showed significant higher angiogenesis.
AB - Angiogenesis is a process of formation of new small blood vessels from existing vessels and is very critical for proper and rapid tissue healing. As reactive oxygen species (ROS) are known for their ability to promote angiogenesis, in this study zinc oxide (ZnO) and zinc peroxide (ZnO2) nano-particles (NP's) encapsulated chitosan (CS) and cellulose based hydrogels were synthesized. ZnO2 is comparatively stronger oxidizing agent as compared to ZnO, therefore, in this study it was hypothesized that ZnO2 would deliver better angiogenic potential than ZnO. Three types of hydrogels were prepared; control hydrogel (without nano-particles), hydrogel having ZnO NP's and hydrogel having ZnO2 NP's. The hydrogels were characterized by scanning electron microscopy (SEM) for structural morphology, Fourier transform infrared spectroscopy (FTIR) for chemical functional groups analyses and X-ray Diffraction (XRD) to investigate the crystalline or amorphous structure of NP's. The solution absorption capacity was tested in PBS and degradation was investigated in PBS, PBS/H2O2 and PBS/lysozyme solutions. The angiogenic behavior of these materials was studied in chorioallantoic membrane (CAM) assay and ZnO2 based material showed significant higher angiogenesis.
KW - Angiogenesis
KW - Chorioallantoic membrane assay
KW - Zinc oxide nanoparticles
KW - Zinc peroxide nanoparticles
KW - Reactive oxygen species
U2 - 10.1016/j.matdes.2017.07.023
DO - 10.1016/j.matdes.2017.07.023
M3 - Journal article
VL - 132
SP - 409
EP - 418
JO - Materials and Design
JF - Materials and Design
SN - 0264-1275
ER -