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    Rights statement: This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 184, 2017 DOI: 10.1016/j.chemosphere.2017.05.159

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A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples

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A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples. / Jiang, Bo; Li, Guanghe; Xing, Yi; Zhang, Dayi; Jia, Jianli; Cui, Zhisong; Luan, Xiao; Tang, Hui.

In: Chemosphere, Vol. 184, 10.2017, p. 384-392.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jiang, B, Li, G, Xing, Y, Zhang, D, Jia, J, Cui, Z, Luan, X & Tang, H 2017, 'A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples', Chemosphere, vol. 184, pp. 384-392. https://doi.org/10.1016/j.chemosphere.2017.05.159

APA

Jiang, B., Li, G., Xing, Y., Zhang, D., Jia, J., Cui, Z., Luan, X., & Tang, H. (2017). A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples. Chemosphere, 184, 384-392. https://doi.org/10.1016/j.chemosphere.2017.05.159

Vancouver

Author

Jiang, Bo ; Li, Guanghe ; Xing, Yi ; Zhang, Dayi ; Jia, Jianli ; Cui, Zhisong ; Luan, Xiao ; Tang, Hui. / A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples. In: Chemosphere. 2017 ; Vol. 184. pp. 384-392.

Bibtex

@article{3f7a64ca96f049318f105811f61132a2,
title = "A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples",
abstract = "Whole-cell bioreporters have emerged as promising tools for genotoxicity evaluation, due to their rapidity, cost-effectiveness, sensitivity and selectivity. In this study, a method for detecting genotoxicity in environmental samples was developed using the bioluminescent whole-cell bioreporter Escherichia coli recA::luxCDABE. To further test its performance in a real world scenario, the E. coli bioreporter was applied in two cases: i) soil samples collected from chromium(VI) contaminated sites; ii) crude oil contaminated seawater collected after the Jiaozhou Bay oil spill which occurred in 2013. The chromium(VI) contaminated soils were pretreated by water extraction, and directly exposed to the bioreporter in two phases: aqueous soil extraction (water phase) and soil supernatant (solid phase). The results indicated that both extractable and soil particle fixed chromium(VI) were bioavailable to the bioreporter, and the solid-phase contact bioreporter assay provided a more precise evaluation of soil genotoxicity. For crude oil contaminated seawater, the response of the bioreporter clearly illustrated the spatial and time change in genotoxicity surrounding the spill site, suggesting that the crude oil degradation process decreased the genotoxic risk to ecosystem. In addition, the performance of the bioreporter was simulated by a modified cross-regulation gene expression model, which quantitatively described the DNA damage response of the E. coli bioreporter. Accordingly, the bioluminescent response of the bioreporter was calculated as the mitomycin C equivalent, enabling quantitative comparison of genotoxicities between different environmental samples. This bioreporter assay provides a rapid and sensitive screening tool for direct genotoxicity assessment of environmental samples.",
keywords = "Genotoxicity, Seawater, Soil, Simulation, SOS model, Whole-cell bioreporter",
author = "Bo Jiang and Guanghe Li and Yi Xing and Dayi Zhang and Jianli Jia and Zhisong Cui and Xiao Luan and Hui Tang",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 184, 2017 DOI: 10.1016/j.chemosphere.2017.05.159",
year = "2017",
month = oct,
doi = "10.1016/j.chemosphere.2017.05.159",
language = "English",
volume = "184",
pages = "384--392",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "NLM (Medline)",

}

RIS

TY - JOUR

T1 - A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples

AU - Jiang, Bo

AU - Li, Guanghe

AU - Xing, Yi

AU - Zhang, Dayi

AU - Jia, Jianli

AU - Cui, Zhisong

AU - Luan, Xiao

AU - Tang, Hui

N1 - This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 184, 2017 DOI: 10.1016/j.chemosphere.2017.05.159

PY - 2017/10

Y1 - 2017/10

N2 - Whole-cell bioreporters have emerged as promising tools for genotoxicity evaluation, due to their rapidity, cost-effectiveness, sensitivity and selectivity. In this study, a method for detecting genotoxicity in environmental samples was developed using the bioluminescent whole-cell bioreporter Escherichia coli recA::luxCDABE. To further test its performance in a real world scenario, the E. coli bioreporter was applied in two cases: i) soil samples collected from chromium(VI) contaminated sites; ii) crude oil contaminated seawater collected after the Jiaozhou Bay oil spill which occurred in 2013. The chromium(VI) contaminated soils were pretreated by water extraction, and directly exposed to the bioreporter in two phases: aqueous soil extraction (water phase) and soil supernatant (solid phase). The results indicated that both extractable and soil particle fixed chromium(VI) were bioavailable to the bioreporter, and the solid-phase contact bioreporter assay provided a more precise evaluation of soil genotoxicity. For crude oil contaminated seawater, the response of the bioreporter clearly illustrated the spatial and time change in genotoxicity surrounding the spill site, suggesting that the crude oil degradation process decreased the genotoxic risk to ecosystem. In addition, the performance of the bioreporter was simulated by a modified cross-regulation gene expression model, which quantitatively described the DNA damage response of the E. coli bioreporter. Accordingly, the bioluminescent response of the bioreporter was calculated as the mitomycin C equivalent, enabling quantitative comparison of genotoxicities between different environmental samples. This bioreporter assay provides a rapid and sensitive screening tool for direct genotoxicity assessment of environmental samples.

AB - Whole-cell bioreporters have emerged as promising tools for genotoxicity evaluation, due to their rapidity, cost-effectiveness, sensitivity and selectivity. In this study, a method for detecting genotoxicity in environmental samples was developed using the bioluminescent whole-cell bioreporter Escherichia coli recA::luxCDABE. To further test its performance in a real world scenario, the E. coli bioreporter was applied in two cases: i) soil samples collected from chromium(VI) contaminated sites; ii) crude oil contaminated seawater collected after the Jiaozhou Bay oil spill which occurred in 2013. The chromium(VI) contaminated soils were pretreated by water extraction, and directly exposed to the bioreporter in two phases: aqueous soil extraction (water phase) and soil supernatant (solid phase). The results indicated that both extractable and soil particle fixed chromium(VI) were bioavailable to the bioreporter, and the solid-phase contact bioreporter assay provided a more precise evaluation of soil genotoxicity. For crude oil contaminated seawater, the response of the bioreporter clearly illustrated the spatial and time change in genotoxicity surrounding the spill site, suggesting that the crude oil degradation process decreased the genotoxic risk to ecosystem. In addition, the performance of the bioreporter was simulated by a modified cross-regulation gene expression model, which quantitatively described the DNA damage response of the E. coli bioreporter. Accordingly, the bioluminescent response of the bioreporter was calculated as the mitomycin C equivalent, enabling quantitative comparison of genotoxicities between different environmental samples. This bioreporter assay provides a rapid and sensitive screening tool for direct genotoxicity assessment of environmental samples.

KW - Genotoxicity

KW - Seawater

KW - Soil

KW - Simulation

KW - SOS model

KW - Whole-cell bioreporter

U2 - 10.1016/j.chemosphere.2017.05.159

DO - 10.1016/j.chemosphere.2017.05.159

M3 - Journal article

VL - 184

SP - 384

EP - 392

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -