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A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion.

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A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion. / Blunt, Tracy; Taccioli, Guillermo E.; Priestley, Anne et al.
In: Genomics, Vol. 30, No. 2, 11.1995, p. 320-328.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Blunt, T, Taccioli, GE, Priestley, A, Hafezparast, M, McMillan, TJ, Liu, J, Cole, CC, White, J, Alt, FW, Jackson, SP, Schurr, E, Lehmann, AR & Jeggo, PA 1995, 'A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion.', Genomics, vol. 30, no. 2, pp. 320-328. https://doi.org/10.1006/geno.1995.9871

APA

Blunt, T., Taccioli, G. E., Priestley, A., Hafezparast, M., McMillan, T. J., Liu, J., Cole, C. C., White, J., Alt, F. W., Jackson, S. P., Schurr, E., Lehmann, A. R., & Jeggo, P. A. (1995). A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion. Genomics, 30(2), 320-328. https://doi.org/10.1006/geno.1995.9871

Vancouver

Blunt T, Taccioli GE, Priestley A, Hafezparast M, McMillan TJ, Liu J et al. A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion. Genomics. 1995 Nov;30(2):320-328. doi: 10.1006/geno.1995.9871

Author

Blunt, Tracy ; Taccioli, Guillermo E. ; Priestley, Anne et al. / A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion. In: Genomics. 1995 ; Vol. 30, No. 2. pp. 320-328.

Bibtex

@article{528a765ca8f54f719f2ec9f9281deb04,
title = "A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion.",
abstract = "The Chinese hamster ovaryxrsmutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33–q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred toxrscells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines.",
author = "Tracy Blunt and Taccioli, {Guillermo E.} and Anne Priestley and Majid Hafezparast and McMillan, {Trevor J.} and Jing Liu and Cole, {Charlotte C.} and Jacqueline White and Alt, {Frederick W.} and Jackson, {Stephen P.} and Erwin Schurr and Lehmann, {Alan R.} and Jeggo, {Penny A.}",
year = "1995",
month = nov,
doi = "10.1006/geno.1995.9871",
language = "English",
volume = "30",
pages = "320--328",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion.

AU - Blunt, Tracy

AU - Taccioli, Guillermo E.

AU - Priestley, Anne

AU - Hafezparast, Majid

AU - McMillan, Trevor J.

AU - Liu, Jing

AU - Cole, Charlotte C.

AU - White, Jacqueline

AU - Alt, Frederick W.

AU - Jackson, Stephen P.

AU - Schurr, Erwin

AU - Lehmann, Alan R.

AU - Jeggo, Penny A.

PY - 1995/11

Y1 - 1995/11

N2 - The Chinese hamster ovaryxrsmutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33–q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred toxrscells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines.

AB - The Chinese hamster ovaryxrsmutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33–q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred toxrscells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines.

U2 - 10.1006/geno.1995.9871

DO - 10.1006/geno.1995.9871

M3 - Journal article

VL - 30

SP - 320

EP - 328

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -